Monoclonal Anti-BRAF Antibody

B-Raf proto-oncogene, serine/threonine kinase
Recommended Applications
Product Description
Monoclonal Antibody against Human BRAF
Alternative Gene Names
BRAF1, RAFB1
Open Datasheet
Price
$505.00
Product Number
AMAb91257
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Show all images
[4 pictures]
Immunohistochemical staining of human testis shows strong cytoplasmic immunoreactivity in seminiferous tubules.
Show all images
[1 pictures]
Western blot analysis in human cell line MOLT-4.

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
B-Raf proto-oncogene, serine/threonine kinase
Target Gene
BRAF
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000002413 (93%)

Rat ENSRNOG00000010957 (92%)

Clonality
Monoclonal
Clone ID
CL4003
Isotype
IgG1
Host
Mouse
Buffer
The antibodies are delivered in 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
The antibody solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:200 - 1:500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)
All IHC characterization data in 44 normal tissues and 20 cancers for AMAb91257 on the Human Protein Atlas
Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Protein Name
B-Raf proto-oncogene, serine/threonine kinase
Gene Name
BRAF
Alternative Gene Names
BRAF1, RAFB1
UniProt ID
P15056
Gene (Ensembl)
ENSG00000157764
Entrez Gene ID
Entrez gene: 673
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Did we miss your publication?

Have you published using AMAb91257? Please let us know and we will be happy to include your reference on this page.

Read more

Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

Read more

Alternative Antibodies
Anti-BRAF (AMAb91258)
Anti-BRAF (HPA001328)
Anti-BRAF (HPA071048)
Corresponding Antigens
PrEST Antigen BRAF (APrEST79898)

Questions?

Our customer support and expert scientific support teams are here to help you succeed in your research.

Get in touch