Enhanced Validation

Monoclonal Anti-BCL2 Antibody

Bcl2, apoptosis regulator
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Monoclonal Antibody against Human BCL2
Alternative Gene Names
Bcl-2, PPP1R50
Open Datasheet
Price
$505.00
Product Number
AMAb91492
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Show all images
[7 pictures]
Immunohistochemistry analysis in human thyroid gland and liver tissues using AMAb91492 antibody. Corresponding BCL2 RNA-seq data are presented for the same tissues.
Show all images
[1 pictures]
Western blot analysis in human cell line HDLM-2.

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
Bcl2, apoptosis regulator
Target Gene
BCL2
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000057329 (100%)

Rat ENSRNOG00000002791 (94%)

Clonality
Monoclonal
Clone ID
CL7935
Isotype
IgG2a
Host
Mouse
Buffer
The antibodies are delivered in 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
The antibody solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:500 - 1:1000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb91492 on the Human Protein Atlas
Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Protein Name
Bcl2, apoptosis regulator
Gene Name
BCL2
Alternative Gene Names
Bcl-2, PPP1R50
UniProt ID
P10415
Gene (Ensembl)
ENSG00000171791
Entrez Gene ID
Entrez gene: 596
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-BCL2 (HPA055295)

Questions?

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