Enhanced Validation

Monoclonal Anti-ATAD2 Antibody

ATPase family, AAA domain containing 2
References (2)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Genetic validation in WB by siRNA knockdown.
Product Description
Monoclonal Antibody against Human ATAD2
Alternative Gene Names
DKFZp667N1320, MGC29843, MGC5254, PRO2000
Open Datasheet
Price
$505.00
Product Number
AMAb90541
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[5 pictures]
Immunohistochemistry analysis in human testis and skeletal muscle tissues using AMAb90541 antibody. Corresponding ATAD2 RNA-seq data are presented for the same tissues.
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[2 pictures]
Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ATAD2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
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[1 pictures]
Immunofluorescence staining of MCF7 cells using the Anti-ATAD2 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
ATPase family, AAA domain containing 2
Target Gene
ATAD2
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000022360 (82%)

Rat ENSRNOG00000025604 (82%)

Clonality
Monoclonal
Clone ID
CL0182
Isotype
IgG1
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:500 - 1:1000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb90541 on the Human Protein Atlas
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb90541 on the Human Protein Atlas
Protein Name
ATPase family, AAA domain containing 2
Gene Name
ATAD2
Alternative Gene Names
DKFZp667N1320, MGC29843, MGC5254, PRO2000
UniProt ID
Q6PL18
Gene (Ensembl)
ENSG00000156802
Entrez Gene ID
Entrez gene: 29028
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Liu N, Funasaka K, Obayashi T, Miyahara R, Hirooka Y, Goto H, Senga T

ATAD2 is associated with malignant characteristics of pancreatic cancer cells

Oncol Lett , 2019 Jan 23; 17(3):3489-3494. Epub 2019 Jan 23

PubMed ID: 30867788 DOI: 10.3892/ol.2019.9960

Wan WN, Zhang YX, Wang XM, Liu YJ, Zhang YQ, Que YH, Zhao WJ

ATAD2 is highly expressed in ovarian carcinomas and indicates poor prognosis.

Asian Pac J Cancer Prev , 2014; 15(6):2777-83

PubMed ID: 24761900 DOI: None

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-ATAD2 (HPA029424)
Corresponding Antigens
PrEST Antigen ATAD2 (APrEST74649)

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