Monoclonal Anti-ARID1A Antibody

AT rich interactive domain 1A (SWI-like)
Recommended Applications
Product Description
Monoclonal Antibody against Human ARID1A
Alternative Gene Names
B120, BAF250, BAF250a, C10rf4, C1orf4, P270, SMARCF1
Open Datasheet
Price
$505.00
Product Number
AMAb91192
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Show all images
[6 pictures]
Immunohistochemical staining of human Fallopian tube shows strong nuclear positivity in glandular cells.
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[1 pictures]
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY416884)
Show all images
[1 pictures]
Immunofluorescence staining of A-431 cells using the Anti-ARID1A monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
AT rich interactive domain 1A (SWI-like)
Target Gene
ARID1A
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000007880 (95%)

Rat ENSRNOG00000006137 (88%)

Clonality
Monoclonal
Clone ID
CL3595
Isotype
IgG1
Host
Mouse
Buffer
The antibodies are delivered in 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
The antibody solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:50 - 1:200

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)
All IHC characterization data in 44 normal tissues and 20 cancers for AMAb91192 on the Human Protein Atlas
Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb91192 on the Human Protein Atlas
Protein Name
AT rich interactive domain 1A (SWI-like)
Gene Name
ARID1A
Alternative Gene Names
B120, BAF250, BAF250a, C10rf4, C1orf4, P270, SMARCF1
UniProt ID
O14497
Gene (Ensembl)
ENSG00000117713
Entrez Gene ID
Entrez gene: 8289
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.

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Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-ARID1A (HPA005456)
Corresponding Antigens
PrEST Antigen ARID1A (APrEST70748)

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