Enhanced Validation

Monoclonal Anti-ANLN Antibody

anillin, actin binding protein
References (3)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Genetic validation in WB by siRNA knockdown.
Product Description
Monoclonal Antibody against Human ANLN
Alternative Gene Names
ANILLIN, scra, Scraps
Open Datasheet
Price
$505.00
Product Number
AMAb90662
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[6 pictures]
Immunohistochemistry analysis in human testis and skeletal muscle tissues using AMAb90662 antibody. Corresponding ANLN RNA-seq data are presented for the same tissues.
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[2 pictures]
Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ANLN antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
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[5 pictures]
Immunofluorescence staining in HeLa cell line with Anti-ANLN monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
anillin, actin binding protein
Target Gene
ANLN
Belongs to Antibody Panel
Organelle Markers
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000036777 (76%)

Rat ENSRNOG00000014343 (76%)

Clonality
Monoclonal
Clone ID
CL0303
Isotype
IgG1
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:50 - 1:200

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb90662 on the Human Protein Atlas
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb90662 on the Human Protein Atlas
Protein Name
anillin, actin binding protein
Gene Name
ANLN
Alternative Gene Names
ANILLIN, scra, Scraps
UniProt ID
Q9NQW6
Gene (Ensembl)
ENSG00000011426
Entrez Gene ID
Entrez gene: 54443
Shipping
Normally shipped at ambient temperature.
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Mund A, Coscia F, Kriston A, Hollandi R, Kovács F, Brunner AD, Migh E, Schweizer L, Santos A, Bzorek M, Naimy S, Rahbek-Gjerdrum LM, Dyring-Andersen B, Bulkescher J, Lukas C, Eckert MA, Lengyel E, Gnann C, Lundberg E, Horvath P, Mann M

Deep Visual Proteomics defines single-cell identity and heterogeneity

Nat Biotechnol , 2022 May 19; 40(8):1231-1240. Epub 2022 May 19

PubMed ID: 35590073 DOI: 10.1038/s41587-022-01302-5

Lian YF, Huang YL, Wang JL, Deng MH, Xia TL, Zeng MS, Chen MS, Wang HB, Huang YH

Anillin is required for tumor growth and regulated by miR-15a/miR-16-1 in HBV-related hepatocellular carcinoma

Aging (Albany NY) , 2018 Aug 9; 10(8):1884-1901. Epub 2018 Aug 9

PubMed ID: 30103211 DOI: 10.18632/aging.101510

Idichi T, Seki N, Kurahara H, Yonemori K, Osako Y, Arai T, Okato A, Kita Y, Arigami T, Mataki Y, Kijima Y, Maemura K, Natsugoe S

Regulation of actin-binding protein ANLN by antitumor miR-217 inhibits cancer cell aggressiveness in pancreatic ductal adenocarcinoma

Oncotarget , 2017 May 29; 8(32):53180-53193. Epub 2017 May 29

PubMed ID: 28881803 DOI: 10.18632/oncotarget.18261

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-ANLN (AMAb90660)
Anti-ANLN (HPA005680)
Anti-ANLN (HPA050556)
Corresponding Antigens
PrEST Antigen ANLN (APrEST70193)

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