Human Protein Atlas
Dilution: 1:50 - 1:200
Retrieval method: HIER pH6
Working concentration: 1 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 2-10 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Genetic validation in WB by siRNA knockdown.
Anillin is required for tumor growth and regulated by miR-15a/miR-16-1 in HBV-related hepatocellular carcinoma
Aging (Albany NY) , 2018 Aug 9; 10(8):1884-1901. Epub 2018 Aug 9
Regulation of actin-binding protein ANLN by antitumor miR-217 inhibits cancer cell aggressiveness in pancreatic ductal adenocarcinoma
Oncotarget , 2017 May 29; 8(32):53180-53193. Epub 2017 May 29
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