Lane 2: Human cell line U-251 MG
Human Protein Atlas
Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000036777 (76%)
Rat ENSRNOG00000014343 (76%)
Dilution: 1:50 - 1:200
Retrieval method: HIER pH6
Working concentration: 1 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 2-10 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validated in Western blot using relevant lysates (see Western blot application images above)
ANLN Enhances Triple-Negative Breast Cancer Stemness Through TWIST1 and BMP2 and Promotes its Spheroid Growth
Front Mol Biosci , 2021 Jul 1; 8:700973. Epub 2021 Jul 1
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