Enhanced Validation

Monoclonal Anti-ADAR Antibody

adenosine deaminase, RNA-specific
References (3)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Genetic validation in WB by siRNA knockdown.
Product Description
Monoclonal Antibody against Human ADAR
Alternative Gene Names
ADAR1, G1P1, IFI4
Open Datasheet
Price
$505.00
Product Number
AMAb90535
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[5 pictures]
Immunohistochemistry analysis in human cerebral cortex and skeletal muscle tissues using AMAb90535 antibody. Corresponding ADAR RNA-seq data are presented for the same tissues.
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[1 pictures]
Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ADAR antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
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[1 pictures]
Immunofluorescence staining of A549 cells using the Anti-ADAR monoclonal antibody, showing specific staining in the nucleoplasm and nucleoli in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
adenosine deaminase, RNA-specific
Target Gene
ADAR
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000027951 (86%)

Rat ENSRNOG00000020744 (85%)

Clonality
Monoclonal
Clone ID
CL0176
Isotype
IgG1
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:500 - 1:1000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb90535 on the Human Protein Atlas
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb90535 on the Human Protein Atlas
Protein Name
adenosine deaminase, RNA-specific
Gene Name
ADAR
Alternative Gene Names
ADAR1, G1P1, IFI4
UniProt ID
P55265
Gene (Ensembl)
ENSG00000160710
Entrez Gene ID
Entrez gene: 103
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Soler M, Davalos V, Sánchez‐Castillo A, Mora‐Martinez C, Setién F, Siqueira E, Castro de Moura M, Esteller M, Guil S

The transcribed ultraconserved region uc.160+ enhances processing and A‐to‐I editing of the miR‐376 cluster: hypermethylation improves glioma prognosis

Mol Oncol , 2021 Nov 3; 16(3):648-664. Epub 2021 Nov 3

PubMed ID: 34665919 DOI: 10.1002/1878-0261.13121

Altadill T, Dowdy TM, Gill K, Reques A, Menon SS, Moiola CP, Lopez-Gil C, Coll E, Matias-Guiu X, Cabrera S, Garcia A, Reventos J, Byers SW, Gil-Moreno A, Cheema AK, Colas E

Metabolomic and Lipidomic Profiling Identifies The Role of the RNA Editing Pathway in Endometrial Carcinogenesis

Sci Rep , 2017 Aug 18; 7:8803. Epub 2017 Aug 18

PubMed ID: 28821813 DOI: 10.1038/s41598-017-09169-2

Anadón C, Guil S, Simó-Riudalbas L, Moutinho C, Setien F, Martínez-Cardús A, Moran S, Villanueva A, Calaf M, Vidal A, Lazo PA, Zondervan I, Savola S, Kohno T, Yokota J, Ribas de Pouplana L, Esteller M

Gene Amplification-Associated Overexpression of the RNA Editing Enzyme ADAR1 Enhances Human Lung Tumorigenesis

Oncogene , . Epub 2015 Dec 7

PubMed ID: 26640150 DOI: 10.1038/onc.2015.469

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Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-ADAR (HPA003890)
Anti-ADAR (HPA051519)
Corresponding Antigens
PrEST Antigen ADAR (APrEST86657)

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