Enhanced Validation

Monoclonal Anti-PARP1 Antibody

poly (ADP-ribose) polymerase 1
Recommended Applications
Genetic validation in WB by siRNA knockdown.
Product Description
Monoclonal Antibody against Human PARP1
Alternative Gene Names
ADPRT, PARP, PPOL
Price
$505.00
Product Number
AMAb90959
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Target Protein
poly (ADP-ribose) polymerase 1
Target Gene
PARP1
Belongs to Antibody Panel
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000026496 (95%)

Rat ENSRNOG00000003084 (94%)

Clonality
Monoclonal
Clone ID
CL2220
Isotype
IgG1
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:1000 - 1:2500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Protein Name
poly (ADP-ribose) polymerase 1
Gene Name
PARP1
Alternative Gene Names
ADPRT, PARP, PPOL
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Ambur Sankaranarayana R, Florea A, Allekotte S, Vogg AT, Maurer J, Schäfer L, Bolm C, Terhorst S, Classen A, Bauwens M, Morgenroth A, Mottaghy FM

PARP targeted Auger emitter therapy with [125I]PARPi-01 for triple-negative breast cancer

EJNMMI Res , 2022 Sep 14; 12:60. Epub 2022 Sep 14

PubMed ID: 36104637 DOI: 10.1186/s13550-022-00932-9

Johnson KL, Qi Z, Yan Z, Wen X, Nguyen TC, Zaleta-Rivera K, Chen CJ, Fan X, Sriram K, Wan X, Chen ZB, Zhong S

Revealing protein-protein interactions at the transcriptome scale by sequencing

Mol Cell , 2021 Aug 3; 81(19):4091-4103.e9. Epub 2021 Aug 3

PubMed ID: 34348091 DOI: 10.1016/j.molcel.2021.07.006

Sinha A, Saleh A, Endersby R, Yuan SH, Chokshi CR, Brown KR, Kuzio B, Kauppinen T, Singh SK, Baker SJ, McKinnon PJ, Katyal S

RAD51-Mediated DNA Homologous Recombination Is Independent of PTEN Mutational Status

Cancers (Basel) , 2020 Oct 29; 12(11):3178. Epub 2020 Oct 29

PubMed ID: 33138032 DOI: 10.3390/cancers12113178

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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