Enhanced Validation

Anti-PARP1 Antibody

poly (ADP-ribose) polymerase 1
References (1)
Recommended Applications
Genetic validation in WB by siRNA knockdown.
Product Description
Monoclonal Antibody against Human PARP1
Alternative Gene Names
ADPRT, PARP, PPOL
Open Datasheet
Price
$450.00
Product Number
AMAb90959
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[4 pictures]
Immunohistochemical staining of human tonsil shows strong nuclear positivity in lymphoid cells.
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[1 pictures]
Western blot analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PARP1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
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[5 pictures]
Immunofluorescence staining in HeLa cell line with Anti-PARP1 monoclonal antibody, showing cell cycle dependent nuclear staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
poly (ADP-ribose) polymerase 1
Target Gene
PARP1
Belongs to Antibody Panel
Organelle Markers
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
KGGKVFSATLGLVDIVKGTNSYYKLQLLEDDKENRYWIFRSWGRVGTVIGSNKLEQMPSKEDAIEHFMKLYEEKTGNAWHSKNFTKYPKKFYPLEIDYGQDEEAVKKLTVNPGTKSKLPKPVQDLIKMIFDVESMKKAM
Epitope
Binds to an epitope located within the peptide sequence SKLPKPVQDL as determined by overlapping synthetic peptides.
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000026496 (95%)

Rat ENSRNOG00000003084 (94%)

Clonality
Monoclonal
Clone ID
CL2220
Isotype
IgG1
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:1000 - 1:2500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)
All IHC characterization data in 44 normal tissues and 20 cancers for AMAb90959 on the Human Protein Atlas
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb90959 on the Human Protein Atlas
Protein Name
poly (ADP-ribose) polymerase 1
Gene Name
PARP1
Alternative Gene Names
ADPRT, PARP, PPOL
UniProt ID
P09874
Gene (Ensembl)
ENSG00000143799
Entrez Gene ID
Entrez gene: 142
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Sinha A, Saleh A, Endersby R, Yuan SH, Chokshi CR, Brown KR, Kuzio B, Kauppinen T, Singh SK, Baker SJ, McKinnon PJ, Katyal S

RAD51-Mediated DNA Homologous Recombination Is Independent of PTEN Mutational Status

Cancers (Basel) , 2020 Oct 29; 12(11):3178. Epub 2020 Oct 29

PubMed ID: 33138032 DOI: 10.3390/cancers12113178

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Alternative Antibodies
Anti-PARP1 (AMAb90960)
Anti-PARP1 (HPA045168)
Corresponding Antigens
PrEST Antigen PARP1 (APrEST71075)

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