AtlasPlex: Custom Multiplex Immunohistochemistry (IHC) Kit

Visualize More with Flexible Multiplex IHC Panels

AtlasPlex at a glance:

Up to 5-plex staining, completed in a single day
No secondary antibodies, no species-matching headaches
No fixed marker panels — build your own custom 3- or 5-plex
Choose from 17,000+ IHC-validated antibodies covering 12,000+ human genes, all from one supplier, in one kit
Brilliant, reproducible results backed by the Human Protein Atlas' catalog flexibility

How it works:

1) Marker Panel: Build your own from 12,000+ targets

AtlasPlex lets you build a custom multiplex IHC kit by selecting from over 12,000 validated targets. Every antibody is rigorously IHC-validated, backed by extensive Human Protein Atlas tissue and organ images, so you can trust your detection with confidence.

• No primary antibody host species restrictions
• Bright, permanent tyramide signal that won't fade over time
• Low background, high specificity
• Fully compatible with standard FFPE tissue workflows
• Ideal for spatial biology, tumor microenvironment, and biomarker discovery

2) Workflow: from sample to results in one day

AtlasPlex enables iterative multiplexed immunohistochemistry (mIHC) for 3-5 proteins detection in FFPE tissue.

1. Smart Complexing: Biotinylated primary antibodies (Ab1) are pre-bound to HRP, setting the stage for powerful signal amplification.
2. Targeted Tissue Binding: Apply the Ab1-HRP complex directly to fixed tissue. Binding is precise, reliable, and reproducible. 
3. Amplification: Tyramide-based signal amplification deposits a bright, permanent fluorescent signal at each target site, ensuring crisp, high-contrast images. 
4. Enzyme Quenching: Deactivate HRP quickly and cleanly. This rapid, gentle step completely replaces the need for harsh, tissue-damaging antibody stripping, ensuring no interference in downstream rounds. 
5. Repeat: Run multiple antibody cycles (Ab2...Ab5) with only 1.5 hours per cycle. Up to 5-plex per day. 
6. Brilliant Imaging: Capture your results using standard fluorescence microscopy. Reveal distinct biomarker landscapes with unmatched clarity.

3) Imaging: clear spectral coverage for enhanced visualization

4) Advantages: less time & enhanced sensitivity

• Simplify Setup & Save Weeks
  Skip assembling protocols from scratch. AtlasPlex delivers everything in one box, pre-biotinylated antibodies and optimized TSA reagents included, cutting setup and optimization time dramatically.
• Superior Sensitivity for Low-Abundance Proteins
  Detect even the most challenging, low-expression targets. AtlasPlex uses powerful TSA amplification with an integrated quencher to maximize signal-to-noise ratio.
• Unprecedented HPA Flexibility
  No pre-set panels required. AtlasPlex gives you access to a wide range of IHC-validated antibodies from the Human Protein Atlas, covering most human genes.
• Results in a Day: Confident 5-Plex Staining
  Get more from your precious paraffin-embedded sections. AtlasPlex is optimized for robust multiplexing, clearly visualizing up to 5 targets in a single day.

Ready to Transform Your Multiplex Tissue Imaging?

🎯 AtlasPlex: Your Complete Solution for Brilliant, Reproducible Multiplex Tissue Staining

Build Your Kit →

AtlasPlex Technical Questions Answered