AtlasPlex at a glance:
"AtlasPlex allowed us to obtain a multiplexed histological image of our lung alveolar in-vitro model, showing cell positions relative to the fibronectin FN-silk membrane. Combining all stains on one slide cut sample prep time, and Atlas Antibodies’ wide antibody protein coverage enabled us to choose nearly any human protein target."
My Hedhammar,
Professor of Protein Technology, Royal Institute of Technology (KTH), Stockholm
"In our lab we work with tissue that is often irreplaceable. Every antigen retrieval and boiling cycle we can spare matters, because it means we preserve tissue integrity and can get more informative data out of a single, precious section. AtlasPlex's gentler workflow lets us extract far more from these limited samples than a traditional multiplex protocol would allow."
Cecilia Lindskog,
Group leader, Uppsala University / Human Protein Atlas
1) Marker Panel: Build your own from 12,000+ targets
AtlasPlex lets you build a custom multiplex IHC kit by selecting from over 12,000 validated targets. Every antibody is rigorously IHC-validated, backed by extensive Human Protein Atlas tissue and organ images, so you can trust your detection with confidence.
2) Workflow: from sample to results in one day
AtlasPlex enables iterative multiplexed immunohistochemistry (mIHC) for 3-5 proteins detection in FFPE tissue.
3) Imaging: clear spectral coverage for enhanced visualization
AtlasPlex (3 Plex) included in the Kit
AtlasPlex (5 Plex) included in the Kit
4) Advantages: less time & enhanced sensitivity
Is AtlasPlex beginner-friendly?
Yes, AtlasPlex is user-friendly and suitable for beginners, with ready-to-use kits that reduce the need for extensive protocol optimization.
What problem does it solve?
Multiplex IHC is normally weeks of optimization: species-matching secondaries, titrating stripping conditions, validating that harsh stripping didn’t destroy epitopes. AtlasPlex removes those steps. Enzyme quenching replaces antibody stripping entirely, and the kit arrives with reagents already optimized. It also solves the precious-sample problem: everything on one slide instead of serial sections, which matters for biopsies and rare tissue.
What benefits will I actually get?
Up to 5-plex in a single day, at 1.5 hours per antibody cycle. TSA amplification for low-abundance targets. Permanent tyramide signal that won’t fade, so slides can be re-imaged later. And spatial context — cell positions relative to each other and to structures — that single-plex serial sections can’t give you.
How do I use it?
Standard FFPE workflow. Pick your 3–5 targets, we build the kit, you run iterative cycles: apply Ab-HRP complex, TSA amplification, quench, repeat. Image on your existing wide-field scope. No new instrument, no new software.
How does AtlasPlex address workflow/reproducibility issues?
The TSA and quencher system removes the need for secondary antibodies, enhancing reproducibility and streamlining workflows compared to indirect IF.
How long does an experiment take?
All antibodies can be applied in a single day without elution, at 1.5 hours per antibody cycle. Significantly shortening the overall experiment time.
How can we easily find ready-to-use kits with the right panel configuration for our targets?
AtlasPlex allows free panel customization from 12,000+ human genes, making target-specific kit creation simple.
What if I have questions?
Panel design support from the manufacturer’s own scientists. We made the antibodies, so target selection advice comes from the source. Datafile with full specs available before purchase.