PrecisA Monoclonal Antibodies, precise, accurate and targeted mouse monoclonals

PrecisA Monoclonals™ are in-house developed mouse monoclonal antibodies for dedicated targets. The antibodies are validated for use in IHC, WB and ICC-IF.

PrecisA Monoclonals, cultured in murine cell lines, are precise, accurate, and targeted. At Atlas Antibodies we take special care in designing clones that recognize unique non-overlapping epitopes and/or isotypes. As a result, PrecisA Monoclonals offer premium performance in the approved applications.

Using our stringent production process and characterization procedures, PrecisA Monoclonals ensure specificity, multiplexing opportunities, secured continuity, and a stable supply.

Antigen and Clone Selection 

Antigen selection: The Protein Epitope Signature Tag (PrEST) concept gives antibody performance built-in from the start. A proprietary bioinformatics software selects 50-150 amino acid regions with the lowest possible sequence identity to other human proteins. They are then cloned and recombinantly produced in a tightly controlled setup.

Clone selection: Functional characterization is performed on a large number of ELISA positive cell supernatants to select the optimal clones for each application prior to subcloning and expansion of selected Hybridomas. 

Isotyping and Epitope Mapping

Isotyping: All PrecisA Monoclonal antibodies are isotyped to allow multiplexing using isotype-specific secondary antibodies.

Epitope mapping: PrecisA Monoclonals are epitope-mapped using synthetic overlapping peptides in a bead-based array format to select clones with non-overlapping epitopes only. Using this method, we can determine the specific binding site of the antibody to its target protein.

Characterization and Validation

The characterization of PrecisA Monoclonals starts with an extensive literature search to select the most accurate, relevant, and clinically significant tissues to use for immunohistochemistry (IHC) characterization. Our product catalog provides IHC application data obtained from several tissue types. 

Positively stained tissue is displayed together with negative control and clinical cancer tissue when relevant.

The characterization for Western blot (WB) data follows that same working procedure and starts with a profound literature search to find the best matching lysate. Each PrecisA Monoclonal antibody is supplied with the most relevant characterization data for its specific target.

Learn how our antibodies are validated