Organelle Markers for Subcellular Localization Studies
Subcellular localization studies are essential for mapping, characterizing, and revealing the role of the protein in cellular processes. The antibodies in our Organelle Marker panel are mouse PrecisA Monoclonal antibodies targeting a selection of subcellular locations.
Our PrecisA Monoclonals antibodies target markers with known locations and can be used as references to stain and confirm the location of the protein you are studying.
The panel members have been selected for high RNA levels in relevant cell lines and are all validated for ICC-IF. Most of them are also validated in IHC and WB. All antibodies belong to the PrecisA Monoclonals™ brand, which means they are developed under stringent conditions, with a secured continuity and stable supply.
The Organelle Marker Panel
In collaboration with the Human Protein Atlas project, we have developed a collection of reference markers targeting different subcellular locations within the cell. The focus from the start was to select targets and develop antibodies to be ideal markers for various organelles.
The panel included 26 antibodies for 15 different subcellular locations. Multiple antibodies targeting the same organelle with different isotypes or epitopes allow you to choose the antibody that best suits your experiment needs and facilitates multiplexing.
Explore Organelle Markers
The complete Organelle Marker panel is presented in our product catalog. Choose between several different antibodies for each organelle.
Example of marker targeting the organelle TUFM
Immunofluorescence on five cell lines
TUFM (Tu Translation Elongation Factor, Mitochondrial) participates in protein translation in mitochondria. Our Anti-TUFM (AMAb90966) PrecisA Monoclonal antibody is a marker for mitochondria which are the organelles in the cytoplasm responsible for energy production in the cell. The antibody is a versatile marker showing distinct specificity for mitochondria in the immunocytochemistry-immunofluorescence (ICC-IF) application. It demonstrates consistent staining in the five human cell lines we tested: HeLa, A-431, MCF7, U2-OS, and U-251 cells.
Immunohistochemistry - immunofluorescence to visualize multiple localizations
We routinely isotype our PrecisA Monoclonals. To visualize multiple locations in one staining, you can combine different isotypes of PrecisA Monoclonals (followed by detection with isotype-specific secondary antibodies). On the antibody product page, you can find the specific isotype. We can assist you in selecting appropriate markers to combine.
The images below display multiplex IHC-IF staining of the human colon using the mitochondrial marker Anti-TUFM (AMAb90966, IgG1) together with the nuclear marker Anti-HNRNPC (AMAb91010, IgG2a) and the plasma membrane marker Anti-EZR (AMAb90979, IgG2b). An overlay of all three channels is shown to the left, indicating mitochondria in green, nuclei in blue and plasma membranes in red in the same image. The remaining three images display individual stainings with the three markers.
Immunohistochemistry
Our PrecisA Monoclonals Organelle Markers are also tested and validated for use in immunohistochemistry (IHC). The images below show IHC staining of human skin, placenta, kidney, and fallopian tube using the Anti-TUFM antibody.
Western Blot
Western blot validation data is also available for most antibodies in the organelle marker panel.
The antibodies in the Organelle Markers panel help detect fractionated cellular proteins. Below is an example where we have fractionated HeLa cells using a cell fractionation kit. Specific organelle marker antibodies were used to detect proteins located in different cellular compartments.
