Organelle Markers for Subcellular Localization Studies

Subcellular localization studies are important for mapping, characterizing and revealing the role of the protein in cellular processes.  The antibodies in our Organelle Marker panel are mouse monoclonal antibodies targeting a selection of subcellular locations. 

The antibodies target markers with known locations and can be used as references to stain and confirm the location of the protein you are studying.

The panel members have been selected for high RNA levels in relevant cell lines and are all validated for ICC-IF. Most of them are also validated in IHC and WB. All antibodies belong to the PrecisA Monoclonals™ brand, which means they are developed under stringent conditions, with a secured continuity and stable supply.

The Organelle Marker Panel

In collaboration with the Human Protein Atlas project, we have developed a collection of reference markers targeting different subcellular locations within the cell. The focus from the start was to select targets and develop antibodies to be ideal markers for different organelles. 

The panel included markers for 15 different subcellular locations, made up by 27 antibodies. Multiple antibodies targeting the same organelle with different isotypes or epitopes allows you to choose the antibody that best suits your experiment, and facilitate multiplexing.

Explore Organelle Markers

Explore Organelle Markers

The complete Organelle Marker panel is presented in our product catalog. Choose between several different antibodies for each organelle.

Browse antibodies

Organelle marker example targeting TUFM

Immunofluorescence on five cell lines

Anti-TUFM (AMAb90966) is a marker for mitochondria in the Organelle Marker panel. The mitochondria are organelles in the cytoplasm responsible for energy production in the cell. The antibody is a versatile marker showing distinct specificity for mitochondria in the immunocytochemistry-immunofluorescence (ICC-IF) application. It demonstrates consistent staining in five different human cell lines tested, HeLa, A-431, MCF7, U2-OS, and U-251 cells. 

Mitochondrial marker Anti-TUFM immunofluorescent staining of mitochondria.
Mitochondrial marker Anti-TUFM immunofluorescent staining of mitochondria in (from the left) HeLa cells, A-431 cells, MCF7 cells, U2-OS cells, and U-251 cells. The antibody staining is indicated by the staining of mitochondria in green. Nuclear staining is indicated in blue and microtubules in red.

Immunohistochemistry - immunofluorescence to visualize multiple localizations

Since we routinely isotype all our PrecisA Monoclonals, we can offer you antibodies of different isotypes. To visualize multiple locations in one staining, it is possible to combine PrecisA Monoclonals of different isotypes followed by detection with isotype-specific secondary antibodies. One the product page for each antibody you can find the specific isotype stated to assist you in selecting appropriate markers to combine.

The images below displays multiplex IHC-IF staining of the human colon using mitochondrial marker Anti-TUFM (AMAb90966, IgG1)together with nuclear marker Anti-HNRNPC (AMAb91010, IgG2a) and plasma membrane marker Anti-EZR (AMAb90979, IgG2b). An overlay of all three channels is shown to the left, indicating mitochondria in green, nuclei in blue and plasma membranes in red in the same image.  The remaining three images display individual stainings with the three markers. 

Staining of mitochondria itochondria using Anti-TUFM (AMAb90966), nuclei using Anti-HNRNPC (AMAb91010) and plasma membranes using Anti-EZR (AMAb90979)
From the left: overlay visualizing mitochondria in green, nuclei in blue and membranes in red. The remaining images show mitochondria using Anti-TUFM (AMAb90966), nuclei using Anti-HNRNPC (AMAb91010) and plasma membranes using Anti-EZR (AMAb90979).

Immunohistochemistry

In addition to being validated for IF, all our Organelle Markers are tested and validated for IHC. The images below show IHC staining of human skin, placenta, kidney and fallopian tube using the Anti-TUFM antibody. 

nti-TUFM (AMAb90966) used for IHC staining of human skin showing granular cytoplasmic immunoreactivity in the epithelial cells.
From the left: Anti-TUFM (AMAb90966) used for IHC staining of human skin showing granular cytoplasmic immunoreactivity in the epithelial cells. Staining of human placenta showing strong cytoplasmic positivity in trophoblasts. Staining of human kidney showing granular cytoplasmic immunoreactivity in renal tubules, and staining of human fallopian tube showing strong cytoplasmic positivity in glandular epithelium cells.

Western Blot  

For most antibodies in the organelle marker panel, western blot validation data is also available. 

The Organelle Markers can also be used as a tool to detect fractionated cellular proteins. Here is an example where we have fractionated HeLa cells using a cell fractionation kit. Specific organelle marker antibodies were used to detect proteins located in different cellular compartments.

Specific organelle marker antibodies were used to detect proteins located in different cellular compartments.
Specific organelle marker antibodies were used to detect proteins located in different cellular compartments. CE: cytoplasmic extract, ME: membrane extract, NE: nuclear extract, CB: chromatin-bound extract, PE: pellet extract