In situ PLA - Application Example
Proteins form the foundation of cellular structure and orchestrate essential functions within living organisms. Engaging in multifaceted interactions, proteins collaborate with diverse molecules, notably with other proteins, to execute their vital roles. These protein-protein interactions underpin a spectrum of cellular processes, encompassing protein modifications, transport mechanisms, signaling cascades, and cell cycling.
The exploration of protein interactions holds paramount importance in elucidating the intricacies of protein functions within the broader context of cell biology, encompassing both normal physiological states and disease conditions.
The proximity ligation assay (PLA) is a technique designed for the in situ study of protein-protein interactions within tissues or cells. This method enables the detection, visualization, and quantification of these interactions, as well as the identification of individual proteins and protein modifications within cellular or tissue sections.