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Immunohistochemistry in Human Tissues

Immunohistochemistry (IHC) is the most widely used technique in histopathological diagnosis and research for the detection of proteins in tissues and cells.

IHC is a powerful tool for visualizing and characterizing the distribution of specific proteins within the intricate microenvironment of tissues and cells. This technique involves the use of primary antibodies enabling the precise localization of target proteins.

Beyond its diagnostic applications in pathology, IHC has become an indispensable asset in scientific research, facilitating the exploration of cellular processes, and molecular pathways, and the identification of potential therapeutic targets.

Tissue Microarrays (TMAs) involve the arrangement of numerous tissue samples on a single microscope slide, allowing simultaneous examination of multiple specimens under identical experimental conditions.

The TMA technology provides an automated array-based high-throughput technique in which as many as 1,000 paraffin-embedded tissue samples can be brought into one paraffin block in an array format. This allows for protein expression profiling on a large scale.

The synergy between Immunohistochemistry and Tissue Microarrays has transformed the landscape of histopathological research, providing a robust platform for large-scale protein profiling studies.

  

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Download the "Immunohistochemistry in Human Tissues" white paper and learn about:

  • Immunohistochemistry and the Human Protein Atlas
  • Tissue and Pathology Atlases
  • Tissue Microarrays
  • Antigen Retrieval and Staining
  • Evaluation and Validation

Immunohistochemistry in Human Tissues