Immunofluorescence cytochemistry (ICC-IF)
Learn how our antibodies are validated in immunocytochemistry, and find the protocols you need to be successful in your experiment.
Protocols and guides
Use these recommended protocols for optimal results in ICC-IF using our antibodies. The protocols are optimized for Triple A Polyclonals and PrecisA Monoclonals.
ICC-IF Standard Protocol
Validation and characterization in ICC-IF
Our antibodies, Triple A Polyclonals and PrecisA Monoclonals are characterized and validated by Immunofluorescence (ICC-IF) staining in human cell lines by confocal microscopy, making it possible to pinpoint the subcellular distribution of the analyzed target proteins.
Selection of cell lines
How do we ensure that the protein is endogenously expressed in the target cell lines, thereby eliminating any false positive staining caused by the artificial overexpression of proteins?
For ICC-IF testing we select the cell line(s) with a proven endogenous expression of the target gene, using the RNA-seq data published on the Subcellular Section of the Human Protein Atlas.
The Cell Line section contains information on genome-wide RNA expression profiles of human protein-coding genes in 1206 human cell lines, including 1132 cancer cell lines.