QPrEST U520 Mass Spectrometry Protein Standard

U5 small nuclear ribonucleoprotein 200 kDa helicase
Recommended Applications
Product Description
Stable isotope-labeled standard for absolute protein quantification of U520.
Lys, Arg 13C and 15N metabolically labeled recombinant protein fragment.
Alternative Gene Names
Activating signal cointegrator 1 complex subunit 3-like 1, BRR2 homolog, U5 snRNP-specific 200 kDa protein
Price
$790.00
Product Number
QPrEST21776
Unit Size
≥ 1 nmol
Concentration
≥5µM
Availability
On Demand
Ships
ETA 6 weeks
Estimated shipping date is November 28th.
Target protein
U5 small nuclear ribonucleoprotein 200 kDa helicase
Target gene
SNRNP200
Sequence With Theoretical Tryptic Peptides
LHPRDIDAFWLQRQLSRFYDDAIVSQKKADEVLEILKTASDDRECENQLVLLLGFNTFDFIKVLRQHRMMILYCTLLASAQSEAEKERIMGKMEADPELSKFLY
External Data for Theoretical Tryptic Peptides
DIDAFWLQR
FYDDAIVSQK
ADEVLEILK
ECENQLVLLLGFNTFDFIK
MMILYCTLLASAQSEAEK
MEADPELSK
Fusion Tag
A purification and quantification tag (QTag) consisting of a hexahistidine (His6) sequence followed by an Albumin Binding Protein (ABP) domain derived from Streptococcal Protein G
Expression Host
E. coli LysA ArgA BL21(DE3)
Purification method
IMAC purification
Notes
Gently mix before use. Avoid repeated freeze-thaw cycles.
Purity
≥90% as determined by Bioanalyzer Protein 230 Purity Assay
Theoretical MW
30 kDa including tags
Isotopic Incorporation
>99%
Unit Size
≥ 1 nmol supplied in 2 aliquotes
Concentration
≥5µM
Concentration Determination
Concentration determined by LC-MS/MS using a highly pure and amino acid analyzed QTag as internal standard for targeted quantification of the heavy labeled QPrEST. (CV%<10)
Formulation
Lyophilized in 100 mM Tris-HCl 5% Trehalose, pH 8.0
Instructions for Reconstitution
Spin vial before opening. Add 100 μL distilled H2O to the vial and vortex for 30 seconds. Spin down. For further dilution see Application Protocol.
Unit Size
≥ 1 nmol
Current Lot
Produced on demand. Estimated shipping date is 6 weeks after placed order. Contact support@atlasantibodies.com for more information.
Concentration
[This part should be hidden or updated after call to Current Batch]
Product Data Sheet
Absolute Quantification of protein using Mass Spectrometry
Protocols
Produced On Demand. Validation data not established.
Protein Name
U5 small nuclear ribonucleoprotein 200 kDa helicase
Alternative Protein Names
Activating signal cointegrator 1 complex subunit 3-like 1, BRR2 homolog, U5 snRNP-specific 200 kDa protein
Gene Name
SNRNP200
Alternative Gene Names
Activating signal cointegrator 1 complex subunit 3-like 1, BRR2 homolog, U5 snRNP-specific 200 kDa protein
UniProt ID
Shipping
Shipped at ambient temperature
Storage
Lyophilized product should be stored at -20°C for up to 1 year.

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