QPrEST PRDX6 Mass Spectrometry Protein Standard

Peroxiredoxin-6
Recommended Applications
Product Description
Stable isotope-labeled standard for absolute protein quantification of PRDX6.
Lys, Arg 13C and 15N metabolically labeled recombinant protein fragment.
Alternative Gene Names
1-Cys peroxiredoxin, 24 kDa protein, Acidic calcium-independent phospholipase A2, Antioxidant protein 2, Liver 2D page spot 40, Non-selenium glutathione peroxidase, Red blood cells page spot 12
Price
$790.00
Product Number
QPrEST24009
Unit Size
≥ 1 nmol
Concentration
≥5µM
Availability
On Demand
Ships
ETA 6 weeks
Estimated shipping date is November 28th.
Target protein
Peroxiredoxin-6
Target gene
PRDX6
Sequence With Theoretical Tryptic Peptides
GGLLLGDVAPNFEANTTVGRIRFHDFLGDSWGILFSHPRDFTPVCTTELGRAAKLAPEFAKRNVKLIALSIDSVEDHLAWSKDINAYNCEEPTEKLPFPIIDDRNRELAILLGMLDPAEKDEKGMPVTARVVFVFGPDKKLKLSILYPA
External Data for Theoretical Tryptic Peptides
FHDFLGDSWGILFSHPR
DFTPVCTTELGR
LAPEFAK
LIALSIDSVEDHLAWSK
DINAYNCEEPTEK
ELAILLGMLDPAEK
GMPVTAR
VVFVFGPDK
Fusion Tag
A purification and quantification tag (QTag) consisting of a hexahistidine (His6) sequence followed by an Albumin Binding Protein (ABP) domain derived from Streptococcal Protein G
Expression Host
E. coli LysA ArgA BL21(DE3)
Purification method
IMAC purification
Notes
Gently mix before use. Avoid repeated freeze-thaw cycles.
Purity
≥90% as determined by Bioanalyzer Protein 230 Purity Assay
Theoretical MW
34 kDa including tags
Isotopic Incorporation
>99%
Unit Size
≥ 1 nmol supplied in 2 aliquotes
Concentration
≥5µM
Concentration Determination
Concentration determined by LC-MS/MS using a highly pure and amino acid analyzed QTag as internal standard for targeted quantification of the heavy labeled QPrEST. (CV%<10)
Formulation
Lyophilized in 100 mM Tris-HCl 5% Trehalose, pH 8.0
Instructions for Reconstitution
Spin vial before opening. Add 100 μL distilled H2O to the vial and vortex for 30 seconds. Spin down. For further dilution see Application Protocol.
Unit Size
≥ 1 nmol
Current Lot
Produced on demand. Estimated shipping date is 6 weeks after placed order. Contact support@atlasantibodies.com for more information.
Concentration
[This part should be hidden or updated after call to Current Batch]
Product Data Sheet
Absolute Quantification of protein using Mass Spectrometry
Protocols
Produced On Demand. Validation data not established.
Protein Name
Peroxiredoxin-6
Alternative Protein Names
1-Cys peroxiredoxin, 24 kDa protein, Acidic calcium-independent phospholipase A2, Antioxidant protein 2, Liver 2D page spot 40, Non-selenium glutathione peroxidase, Red blood cells page spot 12
Gene Name
PRDX6
Alternative Gene Names
1-Cys peroxiredoxin, 24 kDa protein, Acidic calcium-independent phospholipase A2, Antioxidant protein 2, Liver 2D page spot 40, Non-selenium glutathione peroxidase, Red blood cells page spot 12
UniProt ID
Shipping
Shipped at ambient temperature
Storage
Lyophilized product should be stored at -20°C for up to 1 year.
Zeiler M, Straube WL, Lundberg E, Uhlen M, Mann M

A Protein Epitope Signature Tag (PrEST) library allows SILAC-based absolute quantification and multiplexed determination of protein copy numbers in cell lines.

Mol Cell Proteomics , 2012 Mar; 11(3):O111.009613

PubMed ID: 21964433 DOI: 10.1074/mcp.O111.009613

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