QPrEST PFD1 Mass Spectrometry Protein Standard

Prefoldin subunit 1
Recommended Applications
Product Description
Stable isotope-labeled standard for absolute protein quantification of PFD1.
Lys, Arg ¹³C and ¹⁵N metabolically labeled recombinant protein fragment.
Price
$790.00
Product Number
QPrEST21527
Unit Size
≥1 nmol
Concentration
≥5 µM
Availability
On Demand
Ships
ETA 6 weeks
Target Protein
Prefoldin subunit 1
Target Gene
PFDN1
Sequence with Theoretical Peptides
ELQAKVIDTQQKVKLADIQIEQLNRTKKHAHLTDTEIMTLVDETNMYEGVGRMFILQSKEAIHSQLLEKQKIAEEKIKELEQKKSYLERSVKEAEDNIREMLMARRA
External Data for Theoretical Tryptic Peptides
VIDTQQK
LADIQIEQLNR
HAHLTDTEIMTLVDETNMYEGVGR
EAIHSQLLEK
EAEDNIR
Fusion Tag
A purification and quantification tag (QTag) consisting of a hexahistidine (His6) sequence followed by an Albumin Binding Protein (ABP) domain derived from Streptococcal Protein G
Expression Host
E. coli LysA ArgA BL21(DE3)
Purification Method
IMAC purification
Purity
≥90% as determined by Bioanalyzer Protein 230 Purity Assay
Theoretical MW
30 kDa including tags
Isotopic Incorporation
>99%
Unit Size
≥1 nmol supplied in 2 aliquotes
Concentration
≥5 µM
Concentration Determination
Concentration determined by LC-MS/MS using a highly pure, amino acid analyzed internal reference standard (Qtag), CV≤10%.
Formulation
Lyophilized in 100 mM Tris-HCl 5% Trehalose, pH 8.0
Instructions for Reconstitution
Spin vial before opening. Add 100 μL ultrapure H₂O to the vial. Vortex thoroughly and spin down. For further dilution see Application Protocol.
Unit Size
≥1 nmol
Current Lot
Produced on demand. Estimated shipping date is 6 weeks after placed order. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Absolute Quantification of protein using Mass Spectrometry
Protocols
Produced On Demand. Finished product is subjected to quality control to ensure compliance with product specifications.

For peptide data, see links to external databases.
Protein Name
Prefoldin subunit 1
Gene Name
PFDN1
UniProt ID
Shipping
Shipped at ambient temperature
Storage
Lyophilized product should be stored at -20°C. Product can be stored for up to 1 year after production date.<br><br>Reconstituted product can be stored at -20°C for up to 4 weeks.
Zeiler M, Straube WL, Lundberg E, Uhlen M, Mann M

A Protein Epitope Signature Tag (PrEST) library allows SILAC-based absolute quantification and multiplexed determination of protein copy numbers in cell lines.

Mol Cell Proteomics , 2012 Mar; 11(3):O111.009613

PubMed ID: 21964433 DOI: 10.1074/mcp.O111.009613

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