QPrEST NLRC4 Mass Spectrometry Protein Standard

NLR family CARD domain-containing protein 4
Recommended Applications
Product Description
Stable isotope-labeled standard for absolute protein quantification of NLRC4.
Lys, Arg ¹³C and ¹⁵N metabolically labeled recombinant protein fragment.
Alternative Gene Names
CARD, LRR, and NACHT-containing protein, Caspase recruitment domain-containing protein 12, Ice protease-activating factor
Price
$790.00
Product Number
QPrEST37005
Unit Size
≥1 nmol
Concentration
≥5 µM
Availability
Out of Stock
Ships
ETA 6 weeks
Target Protein
NLR family CARD domain-containing protein 4
Target Gene
NLRC4
Sequence with Theoretical Peptides
TDSLGNLKNLTKLIMDNIKMNEEDAIKLAEGLKNLKKMCLFHLTHLSDIGEGMDYIVKSLSSEPCDLEEIQLVSCCLSANAVKILAQNLHNLVKLSILDLSENYLEKDGNEALHELIDRMNVLEQLTALMLPWG
External Data for Theoretical Tryptic Peptides
LIMDNIK
MNEEDAIK
MCLFHLTHLSDIGEGMDYIVK
SLSSEPCDLEEIQLVSCCLSANAVK
ILAQNLHNLVK
LSILDLSENYLEK
DGNEALHELIDR
Fusion Tag
A purification and quantification tag (QTag) consisting of a hexahistidine (His6) sequence followed by an Albumin Binding Protein (ABP) domain derived from Streptococcal Protein G
Expression Host
E. coli LysA ArgA BL21(DE3)
Purification Method
IMAC purification
Purity
≥90% as determined by Bioanalyzer Protein 230 Purity Assay
Theoretical MW
33 kDa including tags
Isotopic Incorporation
>99%
Unit Size
≥1 nmol supplied in 2 aliquotes
Concentration
≥5 µM
Concentration Determination
Concentration determined by LC-MS/MS using a highly pure, amino acid analyzed internal reference standard (Qtag), CV≤10%.
Formulation
Lyophilized in 100 mM Tris-HCl 5% Trehalose, pH 8.0
Instructions for Reconstitution
Spin vial before opening. Add 100 μL ultrapure H₂O to the vial. Vortex thoroughly and spin down. For further dilution see Application Protocol.
Unit Size
≥1 nmol
Current Lot
Produced on demand. Estimated shipping date is 6 weeks after placed order. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Absolute Quantification of protein using Mass Spectrometry
Protocols
Produced On Demand. Finished product is subjected to quality control to ensure compliance with product specifications.

For peptide data, see links to external databases.
Protein Name
NLR family CARD domain-containing protein 4
Alternative Protein Names
CARD, LRR, and NACHT-containing protein, Caspase recruitment domain-containing protein 12, Ice protease-activating factor
Gene Name
NLRC4
Alternative Gene Names
CARD, LRR, and NACHT-containing protein, Caspase recruitment domain-containing protein 12, Ice protease-activating factor
UniProt ID
Shipping
Shipped at ambient temperature
Storage
Lyophilized product should be stored at -20°C. Product can be stored for up to 1 year after production date.<br><br>Reconstituted product can be stored at -20°C for up to 4 weeks.

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