QPrEST GLCM Mass Spectrometry Protein Standard

Glucosylceramidase
References (1)
Recommended Applications
Product Description
Stable isotope-labeled standard for absolute protein quantification of GLCM.
Lys, Arg ¹³C and ¹⁵N metabolically labeled recombinant protein fragment.
Alternative Gene Names
Acid beta-glucosidase, Alglucerase, Beta-glucocerebrosidase, D-glucosyl-N-acylsphingosine glucohydrolase, Imiglucerase
Open Datasheet
Price
$790.00
Product Number
QPrEST22596
Unit Size
≥1 nmol
Concentration
≥5 µM
Availability
On Demand
Ships
ETA 6 weeks
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Recommended for absolute quantification of protein using Mass Spectrometry
Target Protein
Glucosylceramidase
Target Gene
GBA
Sequence with Theoretical Peptides
VRLLMLDDQRLLLPHWAKVVLTDPEAAKYVHGIAVHWYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRGMQYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDSPIIVDITKDTFYKQPMFYHLGHFSK
External Data for Theoretical Tryptic Peptides
LLMLDDQR
PeptideAtlas
LLLPHWAK
PeptideAtlas
VVLTDPEAAK
PeptideAtlas
YVHGIAVHWYLDFLAPAK
PeptideAtlas SRMAtlas
ATLGETHR
PeptideAtlas
LFPNTMLFASEACVGSK
PeptideAtlas SRMAtlas
FWEQSVR
PeptideAtlas SRMAtlas
NFVDSPIIVDITK
PeptideAtlas SRMAtlas
Fusion Tag
A purification and quantification tag (QTag) consisting of a hexahistidine (His6) sequence followed by an Albumin Binding Protein (ABP) domain derived from Streptococcal Protein G
Expression Host
E. coli LysA ArgA BL21(DE3)
Purification Method
IMAC purification
Purity
≥90% as determined by Bioanalyzer Protein 230 Purity Assay
Theoretical MW
35 kDa including tags
Isotopic Incorporation
>99%
Unit Size
≥1 nmol supplied in 2 aliquotes
Concentration
≥5 µM
Concentration Determination
Concentration determined by LC-MS/MS using a highly pure, amino acid analyzed internal reference standard (Qtag), CV≤10%.
Formulation
Lyophilized in 100 mM Tris-HCl 5% Trehalose, pH 8.0
Instructions for Reconstitution
Spin vial before opening. Add 100 μL ultrapure H₂O to the vial. Vortex thoroughly and spin down. For further dilution see Application Protocol. Material Safety Data Sheet
Unit Size
≥1 nmol
Current Lot
Produced on demand. Estimated shipping date is 6 weeks after placed order. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Absolute Quantification of protein using Mass Spectrometry
Protocols
View all protocols
Produced On Demand. Finished product is subjected to quality control to ensure compliance with product specifications.

For peptide data, see links to external databases.
Protein Name
Glucosylceramidase
Alternative Protein Names
Acid beta-glucosidase, Alglucerase, Beta-glucocerebrosidase, D-glucosyl-N-acylsphingosine glucohydrolase, Imiglucerase
Gene Name
GBA
Alternative Gene Names
Acid beta-glucosidase, Alglucerase, Beta-glucocerebrosidase, D-glucosyl-N-acylsphingosine glucohydrolase, Imiglucerase
UniProt ID
P04062
Shipping
Shipped at ambient temperature
Storage
Lyophilized product should be stored at -20°C. Product can be stored for up to 1 year after production date.<br><br>Reconstituted product can be stored at -20°C for up to 4 weeks.
Oeckl P, Weydt P, Thal DR, Weishaupt JH, Ludolph AC, Otto M

Proteomics in cerebrospinal fluid and spinal cord suggests UCHL1, MAP2 and GPNMB as biomarkers and underpins importance of transcriptional pathways in amyotrophic lateral sclerosis.

Acta Neuropathol , 2020 Jan; 139(1):119-134

PubMed ID: 31701227 DOI: 10.1007/s00401-019-02093-x

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