QPrEST DIAP1 Mass Spectrometry Protein Standard

Protein diaphanous homolog 1
Recommended Applications
Product Description
Stable isotope-labeled standard for absolute protein quantification of DIAP1.
Lys, Arg 13C and 15N metabolically labeled recombinant protein fragment.
Alternative Gene Names
Diaphanous-related formin-1
Price
$790.00
Product Number
QPrEST21453
Unit Size
≥ 1 nmol
Concentration
≥5µM
Availability
On Demand
Ships
ETA 6 weeks
Estimated shipping date is November 28th.
Target protein
Protein diaphanous homolog 1
Target gene
DIAPH1
Sequence With Theoretical Tryptic Peptides
SLLDILKRLHDEKEETAGSYDSRNKHEIIRCLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPNMMIDAAKLLSALCILPQPEDMNERVLEAMTERAEMDEVERFQPLLDGLKSGTTIALKVGCLQLINALITPAE
External Data for Theoretical Tryptic Peptides
EETAGSYDSR
TMLETEEGILLLVR
AMDPAVPNMMIDAAK
LLSALCILPQPEDMNER
VLEAMTER
AEMDEVER
FQPLLDGLK
SGTTIALK
Fusion Tag
A purification and quantification tag (QTag) consisting of a hexahistidine (His6) sequence followed by an Albumin Binding Protein (ABP) domain derived from Streptococcal Protein G
Expression Host
E. coli LysA ArgA BL21(DE3)
Purification method
IMAC purification
Notes
Gently mix before use. Avoid repeated freeze-thaw cycles.
Purity
≥90% as determined by Bioanalyzer Protein 230 Purity Assay
Theoretical MW
33 kDa including tags
Isotopic Incorporation
>99%
Unit Size
≥ 1 nmol supplied in 2 aliquotes
Concentration
≥5µM
Concentration Determination
Concentration determined by LC-MS/MS using a highly pure and amino acid analyzed QTag as internal standard for targeted quantification of the heavy labeled QPrEST. (CV%<10)
Formulation
Lyophilized in 100 mM Tris-HCl 5% Trehalose, pH 8.0
Instructions for Reconstitution
Spin vial before opening. Add 100 μL distilled H2O to the vial and vortex for 30 seconds. Spin down. For further dilution see Application Protocol.
Unit Size
≥ 1 nmol
Current Lot
Produced on demand. Estimated shipping date is 6 weeks after placed order. Contact support@atlasantibodies.com for more information.
Concentration
[This part should be hidden or updated after call to Current Batch]
Product Data Sheet
Absolute Quantification of protein using Mass Spectrometry
Protocols
Produced On Demand. Validation data not established.
Protein Name
Protein diaphanous homolog 1
Alternative Protein Names
Diaphanous-related formin-1
Gene Name
DIAPH1
Alternative Gene Names
Diaphanous-related formin-1
UniProt ID
Shipping
Shipped at ambient temperature
Storage
Lyophilized product should be stored at -20°C for up to 1 year.

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