QPrEST CENPF Mass Spectrometry Protein Standard

Centromere protein F
Recommended Applications
Product Description
Stable isotope-labeled standard for absolute protein quantification of CENPF.
Lys, Arg 13C and 15N metabolically labeled recombinant protein fragment.
Alternative Gene Names
AH antigen, Kinetochore protein CENPF, Mitosin
Price
$790.00
Product Number
QPrEST24747
Unit Size
≥ 1 nmol
Concentration
≥5µM
Availability
On Demand
Ships
ETA 6 weeks
Target protein
Centromere protein F
Target gene
CENPF
Sequence With Theoretical Tryptic Peptides
CISELSFSGPNALVPMDFLGNQEDIHNLQLRVKETSNENLRLLHVIEDRDRKVESLLNEMKELDSKLHLQEVQLMTKIEACIELEKIVGELKKENSDLSEKLEYFSCDHQEL
External Data for Theoretical Tryptic Peptides
ETSNENLR
LLHVIEDR
VESLLNEMK
LHLQEVQLMTK
IEACIELEK
ENSDLSEK
Fusion Tag
A purification and quantification tag (QTag) consisting of a hexahistidine (His6) sequence followed by an Albumin Binding Protein (ABP) domain derived from Streptococcal Protein G
Expression Host
E. coli LysA ArgA BL21(DE3)
Purification method
IMAC purification
Notes
Gently mix before use. Avoid repeated freeze-thaw cycles.
Purity
≥90% as determined by Bioanalyzer Protein 230 Purity Assay
Theoretical MW
31 kDa including tags
Isotopic Incorporation
>99%
Unit Size
≥ 1 nmol supplied in 2 aliquotes
Concentration
≥5µM
Concentration Determination
Concentration determined by LC-MS/MS using a highly pure and amino acid analyzed QTag as internal standard for targeted quantification of the heavy labeled QPrEST. (CV%<10)
Formulation
Lyophilized in 100 mM Tris-HCl 5% Trehalose, pH 8.0
Instructions for Reconstitution
Spin vial before opening. Add 100 μL distilled H2O to the vial and vortex for 30 seconds. Spin down. For further dilution see Application Protocol.
Unit Size
≥ 1 nmol
Current Lot
Produced on demand. Estimated shipping date is 6 weeks after placed order. Contact support@atlasantibodies.com for more information.
Concentration
[This part should be hidden or updated after call to Current Batch]
Product Data Sheet
Absolute Quantification of protein using Mass Spectrometry
Protocols
Produced On Demand. Validation data not established.
Protein Name
Centromere protein F
Alternative Protein Names
AH antigen, Kinetochore protein CENPF, Mitosin
Gene Name
CENPF
Alternative Gene Names
AH antigen, Kinetochore protein CENPF, Mitosin
UniProt ID
Shipping
Shipped at ambient temperature
Storage
Lyophilized product should be stored at -20°C for up to 1 year.

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