Anti-ZNF282 Antibody

zinc finger protein 282
References (2)
Recommended Applications
Product Description
Polyclonal Antibody against Human ZNF282
Alternative Gene Names
HUB1
Open Datasheet
Price
$450.00
Product Number
HPA024374
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[4 pictures]
Immunohistochemical staining of human endometrium shows moderate nuclear positivity in glandular cells.
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[1 pictures]
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.

Additional Images

See additional IHC images using this antibody for 44 normal tissues and 20 cancers on the Human Protein Atlas
Target Protein
zinc finger protein 282
Target Gene
ZNF282
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
DIAVYFSEDEWKNLDEWQKELYNNLVKENYKTLMSLDAEGSVPKPDAPVQAEPREEPCVWEQRHPEEREIPMDPEAGAEPLVPAQDASSQVKREDTLCVRGQRGLEERAIPTESITDSPISAQDLLSRIKQEEHQCVWDQQDLA
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000026981 (87%)

Mouse ENSMUSG00000025821 (87%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:200 - 1:500

Retrieval method: HIER pH6

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Protocols
View all protocols
Protein Name
zinc finger protein 282
Gene Name
ZNF282
Alternative Gene Names
HUB1
UniProt ID
Q9UDV7
Gene (Ensembl)
ENSG00000170265
Entrez Gene ID
Entrez gene: 8427
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000170265 on the Human Protein Atlas

Yeo SY, Ha SY, Yu EJ, Lee KW, Kim JH, Kim SH

ZNF282 (Zinc finger protein 282), a novel E2F1 co-activator, promotes esophageal squamous cell carcinoma

Oncotarget , 2014 Dec 15; 5(23):12260-12272. Epub 2014 Oct 24

PubMed ID: 25373738 DOI: 10.18632/oncotarget.2630

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers 50% of the product price as a refund, or 50% discount on your next vial purchase.

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Corresponding Antigens
PrEST Antigen ZNF282 (APrEST70977)

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