Enhanced Validation

Anti-XRCC4 Antibody

X-ray repair complementing defective repair in Chinese hamster cells 4
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Genetic validation in WB by siRNA knockdown.
Product Description
Polyclonal Antibody against Human XRCC4
Price
$445.00
Product Number
HPA006801
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Target Protein
X-ray repair complementing defective repair in Chinese hamster cells 4
Target Gene
XRCC4
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
KCVSAKEALETDLYKRFILVLNEKKTKIRSLHNKLLNAAQEREKDIKQEGETAICSEMTADRDPVYDESTDEESENQTDLSGLASAAVSKDDSIISSLDVTDIAPSRKRRQRMQRNLGTEPKMAPQENQLQEK
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000021615 (67%)

Rat ENSRNOG00000029966 (63%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:200 - 1:500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Recombinant expression validation in WB using target protein overexpression.

Protein Name
X-ray repair complementing defective repair in Chinese hamster cells 4
Gene Name
XRCC4
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000152422 on the Human Protein Atlas

Bezine E, Malaisé Y, Loeuillet A, Chevalier M, Boutet-Robinet E, Salles B, Mirey G, Vignard J

Cell resistance to the Cytolethal Distending Toxin involves an association of DNA repair mechanisms

Sci Rep , 2016 Oct 24; 6:36022. Epub 2016 Oct 24

PubMed ID: 27775089 DOI: 10.1038/srep36022

Reid DA, Keegan S, Leo-Macias A, Watanabe G, Strande NT, Chang HH, Oksuz BA, Fenyo D, Lieber MR, Ramsden DA, Rothenberg E

Organization and dynamics of the nonhomologous end-joining machinery during DNA double-strand break repair

Proc Natl Acad Sci U S A , 2015 May 19; 112(20):E2575-E2584. Epub 2015 May 4

PubMed ID: 25941401 DOI: 10.1073/pnas.1420115112

Stadler C, Rexhepaj E, Singan VR, Murphy RF, Pepperkok R, Uhlén M, Simpson JC, Lundberg E

Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.

Nat Methods , 2013 Apr; 10(4):315-23. Epub 2013 Feb 24

PubMed ID: 23435261 DOI: 10.1038/nmeth.2377

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Alternative Antibodies
Corresponding Antigens

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