Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000021615 (67%)
Rat ENSRNOG00000029966 (63%)
Dilution: 1:200 - 1:500
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Genetic validation in WB by siRNA knockdown.
Recombinant expression validation in WB using target protein overexpression.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Cell resistance to the Cytolethal Distending Toxin involves an association of DNA repair mechanisms
Sci Rep , 2016 Oct 24; 6:36022. Epub 2016 Oct 24
Organization and dynamics of the nonhomologous end-joining machinery during DNA double-strand break repair
Proc Natl Acad Sci U S A , 2015 May 19; 112(20):E2575-E2584. Epub 2015 May 4
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Nat Methods , 2013 Apr; 10(4):315-23. Epub 2013 Feb 24
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