Enhanced Validation

Polyclonal Anti-WIPF1 Antibody

WAS/WASL interacting protein family, member 1
References (1)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Polyclonal Antibody against Human WIPF1
Alternative Gene Names
WASPIP, WIP
Open Datasheet
Price
$505.00
Product Number
HPA003739
Unit Size
Concentration
Lot dependent
Availability
On Demand
Ships
On Demand
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[3 pictures]
Immunohistochemistry analysis in human spleen and pancreas tissues using Anti-WIPF1 antibody. Corresponding WIPF1 RNA-seq data are presented for the same tissues.
Show all images
[1 pictures]
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human cell line HEL

Additional Images

See additional IHC images using this antibody for 44 normal tissues and 20 cancers on the Human Protein Atlas
Target Protein
WAS/WASL interacting protein family, member 1
Target Gene
WIPF1
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
PPPPVSRNGSTSRALPATPQLPSRSGVDSPRSGPRPPLPPDRPSAGAPPPPPPSTSIRNGFQDSPCEDEWESRFYFHPISDLPPPEPYVQTTKSYPSKLARNESRSGSNRRERGAP
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000075284 (92%)

Rat ENSRNOG00000018406 (90%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:50 - 1:200

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for HPA003739 on the Human Protein Atlas
Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Protein Name
WAS/WASL interacting protein family, member 1
Gene Name
WIPF1
Alternative Gene Names
WASPIP, WIP
UniProt ID
O43516
Gene (Ensembl)
ENSG00000115935
Entrez Gene ID
Entrez gene: 7456
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000115935 on the Human Protein Atlas

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Have you published using HPA003739? Please let us know and we will be happy to include your reference on this page.

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-WIPF1 (HPA070706)
Corresponding Antigens
PrEST Antigen WIPF1 (APrEST86504)

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