Lane 2: Human cell line RT-4
Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000031166 (98%)
Rat ENSRNOG00000039587 (98%)
Dilution: 1:500 - 1:1000
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
WD-repeat protein WDR13 is a novel transcriptional regulator of c-Jun and modulates intestinal homeostasis in mice
BMC Cancer , 2017 Feb 21; 17:148. Epub 2017 Feb 21
Implication of Genetic Deletion of Wdr13 in Mice: Mild Anxiety, Better Performance in Spatial Memory Task, with Upregulation of Multiple Synaptic Proteins
Front Mol Neurosci , 2016 Aug 30; 9:73. Epub 2016 Aug 30
Role of mouse Wdr13 in placental growth; a genetic evidence for lifetime body weight determination by placenta during development
Sci Rep , 2015 Aug 26; 5:13371. Epub 2015 Aug 26
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Nat Methods , 2013 Apr; 10(4):315-23. Epub 2013 Feb 24
Lack of Wdr13 Gene in Mice Leads to Enhanced Pancreatic Beta Cell Proliferation, Hyperinsulinemia and Mild Obesity
PLoS One , 2012; 7(6):e38685. Epub 2012 Jun 8
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