Enhanced Validation

Anti-VPS53 Antibody

vacuolar protein sorting 53 homolog (S. cerevisiae)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Polyclonal Antibody against Human VPS53
Alternative Gene Names
FLJ10979, HCCS1
Price
$445.00
Product Number
HPA024446
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Target Protein
vacuolar protein sorting 53 homolog (S. cerevisiae)
Target Gene
VPS53
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
ETFQKILDMKGLKRSEQSSMLELLRQRLPAPPSGAESSGSLSLTAPTPEQESSRIRKLEKLIK
Verified Species Reactivity
Human, Mouse, Rat
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000006895 (94%)

Mouse ENSMUSG00000017288 (92%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:50 - 1:200

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Protein Name
vacuolar protein sorting 53 homolog (S. cerevisiae)
Gene Name
VPS53
Alternative Gene Names
FLJ10979, HCCS1
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000141252 on the Human Protein Atlas

Gershlick DC, Ishida M, Jones JR, Bellomo A, Bonifacino JS, Everman DB

A neurodevelopmental disorder caused by mutations in the VPS51 subunit of the GARP and EARP complexes

Hum Mol Genet , 2019 Jan 8; 28(9):1548-1560. Epub 2019 Jan 8

PubMed ID: 30624672 DOI: 10.1093/hmg/ddy423

Hunter MR, Hesketh GG, Benedyk TH, Gingras AC, Graham SC

Proteomic and Biochemical Comparison of the Cellular Interaction Partners of Human VPS33A and VPS33B

J Mol Biol , 2018 Jul 6; 430(14):2153-2163

PubMed ID: 29778605 DOI: 10.1016/j.jmb.2018.05.019

Gershlick DC, Schindler C, Chen Y, Bonifacino JS

TSSC1 is novel component of the endosomal retrieval machinery

Mol Biol Cell , 2016 Sep 15; 27(18):2867-2878

PubMed ID: 27440922 DOI: 10.1091/mbc.E16-04-0209

Did we miss your publication?

Have you published using HPA024446? Please let us know and we will be happy to include your reference on this page.

Read more

Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers 50% of the product price as a refund, or 50% discount on your next vial purchase.

Read more

Alternative Antibodies
Corresponding Antigens

Questions?

Our customer support and expert scientific support teams are here to help you succeed in your research.

Get in touch