Highest antigen sequence identity to the following orthologs:
Rat ENSRNOG00000018087 (99%)
Mouse ENSMUSG00000026728 (99%)
Dilution: 1:1000 - 1:2500
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
In vitro and in vivo characterization of a recombinant rhesus cytomegalovirus containing a complete genome
PLoS Pathog , 2020 Nov 24; 16(11):e1008666. Epub 2020 Nov 24
FOXK1, Regulated by miR-365-3p, Promotes Cell Growth and EMT Indicates Unfavorable Prognosis in Breast Cancer
Onco Targets Ther , 2020 Jan 21; 13:623-634. Epub 2020 Jan 21
Ginkgolic Acid, a SUMO-1 Inhibitor, Inhibits the Progression of Oral Squamous Cell Carcinoma by Alleviating SUMOylation of SMAD4
Mol Ther Oncolytics , 2019 Dec 14; 16:86-99. Epub 2019 Dec 14
Development of a predictive model for stromal content in prostate cancer samples to improve signature performance
J Pathol , 2019 Oct 16; 249(4):411-424. Epub 2019 Oct 16
PEP06 polypeptide 30 exerts antitumour effect in colorectal carcinoma via inhibiting epithelial–mesenchymal transition
Br J Pharmacol , 2018 Jun 15; 175(15):3111-3130. Epub 2018 Jun 15
2-Arachidonoylglycerol Reduces Proteoglycans and Enhances Remyelination in a Progressive Model of Demyelination
J Neurosci , 2017 Aug 30; 37(35):8385-8398
A 3D in vitro model of the human breast duct: a method to unravel myoepithelial-luminal interactions in the progression of breast cancer
Breast Cancer Res , 2017 Apr 21; 19:50. Epub 2017 Apr 21
Establishment of 3D Co-Culture Models from Different Stages of Human Tongue Tumorigenesis: Utility in Understanding Neoplastic Progression
PLoS One , 2016 Aug 8; 11(8):e0160615. Epub 2016 Aug 8
Extracellular Vesicles from Metastatic Rat Prostate Tumors Prime the Normal Prostate Tissue to Facilitate Tumor Growth
Sci Rep , 2016 Aug 23; 6:31805. Epub 2016 Aug 23
Inhibition of Lysyl Oxidase and Lysyl Oxidase-Like Enzymes Has Tumour-Promoting and Tumour-Suppressing Roles in Experimental Prostate Cancer
Sci Rep , 2016 Jan 25; 6:19608. Epub 2016 Jan 25
Changes in Cannabinoid Receptors, Aquaporin 4 and Vimentin Expression after Traumatic Brain Injury in Adolescent Male Mice. Association with Edema and Neurological Deficit
PLoS One , 2015; 10(6):e0128782. Epub 2015 Jun 3
Regulation of Glutamine Carrier Proteins by RNF5 Determines Breast Cancer Response to ER Stress-inducing Chemotherapies
Cancer Cell , 2015 Mar 9; 27(3):354-369
Tumor suppressive microRNA-138 contributes to cell migration and invasion through its targeting of vimentin in renal cell carcinoma
Int J Oncol , 2012 Sep; 41(3):805-817. Epub 2012 Jul 3
Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy.
J Proteomics , 2012 Apr 3; 75(7):2236-51. Epub 2012 Feb 15
Contribution from Undisclosed customer — April 26, 2018
Western blot using Anti-VIM antibody (HPA001762) in K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line.
Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line.
PVDF membrane was probed with 0.7 ug/mL of Anti-Vim antibody (HPA001762). A specific band was detected for Vimentin at approximately 55 kDa (as indicated) in
the parental K562 cell line whereas in the knockout K562 cell line no band was detected.
The experiment was conducted under reducing conditions.
Join the Explorer Program
Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers 50% of the product price as a refund, or 50% discount on your next vial purchase.