Enhanced Validation

Anti-VIM Antibody

vimentin
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.
Product Description
Polyclonal Antibody against Human VIM
Price
$450.00
Product Number
HPA001762
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Target Protein
vimentin
Target Gene
VIM
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
FANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAENTLQSFRQDVDNASLARLDLERKVES
Verified Species Reactivity
Human, Mouse
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000018087 (99%)

Mouse ENSMUSG00000026728 (99%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:1000 - 1:2500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Western Blot (WB)

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

Protein Name
vimentin
Gene Name
VIM
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000026025 on the Human Protein Atlas

Taher H, Mahyari E, Kreklywich C, Uebelhoer LS, McArdle MR, Moström MJ, Bhusari A, Nekorchuk M, E X, Whitmer T, Scheef EA, Sprehe LM, Roberts DL, Hughes CM, Jackson KA, Selseth AN, Ventura AB, Cleveland-Rubeor HC, Yue Y, Schmidt KA, Shao J, Edlefsen PT, Smedley J, Kowalik TF, Stanton RJ, Axthelm MK, Estes JD, Hansen SG, Kaur A, Barry PA, Bimber BN, Picker LJ, Streblow DN, Früh K, Malouli D

In vitro and in vivo characterization of a recombinant rhesus cytomegalovirus containing a complete genome

PLoS Pathog , 2020 Nov 24; 16(11):e1008666. Epub 2020 Nov 24

PubMed ID: 33232376 DOI: 10.1371/journal.ppat.1008666

Gao F, Tian J

FOXK1, Regulated by miR-365-3p, Promotes Cell Growth and EMT Indicates Unfavorable Prognosis in Breast Cancer

Onco Targets Ther , 2020 Jan 21; 13:623-634. Epub 2020 Jan 21

PubMed ID: 32021304 DOI: 10.2147/OTT.S212702

Liu K, Wang X, Li D, Xu D, Li D, Lv Z, Zhao D, Chu WF, Wang XF

Ginkgolic Acid, a SUMO-1 Inhibitor, Inhibits the Progression of Oral Squamous Cell Carcinoma by Alleviating SUMOylation of SMAD4

Mol Ther Oncolytics , 2019 Dec 14; 16:86-99. Epub 2019 Dec 14

PubMed ID: 31970286 DOI: 10.1016/j.omto.2019.12.005

Boufaied N, Takhar M, Nash C, Erho N, Bismar TA, Davicioni E, Thomson AA

Development of a predictive model for stromal content in prostate cancer samples to improve signature performance

J Pathol , 2019 Oct 16; 249(4):411-424. Epub 2019 Oct 16

PubMed ID: 31206668 DOI: 10.1002/path.5315

Yu S, Li L, Tian W, Nie D, Mu W, Qiu F, Liu Y, Liu X, Wang X, Du Z, Chu W, Yang B

PEP06 polypeptide 30 exerts antitumour effect in colorectal carcinoma via inhibiting epithelial–mesenchymal transition

Br J Pharmacol , 2018 Jun 15; 175(15):3111-3130. Epub 2018 Jun 15

PubMed ID: 29722931 DOI: 10.1111/bph.14352

Feliú A, Bonilla del Río I, Carrillo-Salinas FJ, Hernández-Torres G, Mestre L, Puente N, Ortega-Gutiérrez S, López-Rodríguez ML, Grandes P, Mecha M, Guaza C

2-Arachidonoylglycerol Reduces Proteoglycans and Enhances Remyelination in a Progressive Model of Demyelination

J Neurosci , 2017 Aug 30; 37(35):8385-8398

PubMed ID: 28751457 DOI: 10.1523/JNEUROSCI.2900-16.2017

Carter EP, Gopsill JA, Gomm JJ, Jones JL, Grose RP

A 3D in vitro model of the human breast duct: a method to unravel myoepithelial-luminal interactions in the progression of breast cancer

Breast Cancer Res , 2017 Apr 21; 19:50. Epub 2017 Apr 21

PubMed ID: 28427436 DOI: 10.1186/s13058-017-0843-4

Sawant S, Dongre H, Singh AK, Joshi S, Costea DE, Mahadik S, Ahire C, Makani V, Dange P, Sharma S, Chaukar D, Vaidya M

Establishment of 3D Co-Culture Models from Different Stages of Human Tongue Tumorigenesis: Utility in Understanding Neoplastic Progression

PLoS One , 2016 Aug 8; 11(8):e0160615. Epub 2016 Aug 8

PubMed ID: 27501241 DOI: 10.1371/journal.pone.0160615

Halin Bergström S, Hägglöf C, Thysell E, Bergh A, Wikström P, Lundholm M

Extracellular Vesicles from Metastatic Rat Prostate Tumors Prime the Normal Prostate Tissue to Facilitate Tumor Growth

Sci Rep , 2016 Aug 23; 6:31805. Epub 2016 Aug 23

PubMed ID: 27550147 DOI: 10.1038/srep31805

Nilsson M, Adamo H, Bergh A, Halin Bergström S

Inhibition of Lysyl Oxidase and Lysyl Oxidase-Like Enzymes Has Tumour-Promoting and Tumour-Suppressing Roles in Experimental Prostate Cancer

Sci Rep , 2016 Jan 25; 6:19608. Epub 2016 Jan 25

PubMed ID: 26804196 DOI: 10.1038/srep19608

Lopez-Rodriguez AB, Acaz-Fonseca E, Viveros MP, Garcia-Segura LM

Changes in Cannabinoid Receptors, Aquaporin 4 and Vimentin Expression after Traumatic Brain Injury in Adolescent Male Mice. Association with Edema and Neurological Deficit

PLoS One , 2015; 10(6):e0128782. Epub 2015 Jun 3

PubMed ID: 26039099 DOI: 10.1371/journal.pone.0128782

Jeon YJ, Khelifa S, Ratnikov B, Scott DA, Feng Y, Parisi F, Ruller C, Lau E, Kim H, Brill LM, Jiang T, Rimm D, Cardiff RD, Mills GB, Smith JW, Osterman AL, Kluger Y, Ronai ZA

Regulation of Glutamine Carrier Proteins by RNF5 Determines Breast Cancer Response to ER Stress-inducing Chemotherapies

Cancer Cell , 2015 Mar 9; 27(3):354-369

PubMed ID: 25759021 DOI: 10.1016/j.ccell.2015.02.006

YAMASAKI T, SEKI N, YAMADA Y, YOSHINO H, HIDAKA H, CHIYOMARU T, NOHATA N, KINOSHITA T, NAKAGAWA M, ENOKIDA H

Tumor suppressive microRNA-138 contributes to cell migration and invasion through its targeting of vimentin in renal cell carcinoma

Int J Oncol , 2012 Sep; 41(3):805-817. Epub 2012 Jul 3

PubMed ID: 22766839 DOI: 10.3892/ijo.2012.1543

Stadler C, Hjelmare M, Neumann B, Jonasson K, Pepperkok R, Uhlén M, Lundberg E

Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy.

J Proteomics , 2012 Apr 3; 75(7):2236-51. Epub 2012 Feb 15

PubMed ID: 22361696 DOI: 10.1016/j.jprot.2012.01.030

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User Data, Western Blot KO, Customer Submission

Contribution from Undisclosed customer — April 26, 2018

Western blot using Anti-VIM antibody (HPA001762) in K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line.

hpa001762_wb_ko

Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line.

PVDF membrane was probed with 0.7 ug/mL of Anti-Vim antibody (HPA001762). A specific band was detected for Vimentin at approximately 55 kDa (as indicated) in
the parental K562 cell line whereas in the knockout K562 cell line no band was detected. 

The experiment was conducted under reducing conditions.

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Alternative Antibodies
Corresponding Antigens

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