Enhanced Validation

Anti-VARS Antibody

valyl-tRNA synthetase
Recommended Applications
Genetic validation in WB by siRNA knockdown.
Product Description
Polyclonal Antibody against Human VARS
Alternative Gene Names
VARS2
Price
$450.00
Product Number
HPA046710
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Target Protein
valyl-tRNA synthetase
Target Gene
VARS
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
AVALASDRCSIHLQLQGLVDPARELGKLQAKRVEAQRQAQRLRERRAASGYPVKVPLEVQEADEAKLQQTEAELRKVDEAIALFQKML
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000000867 (93%)

Mouse ENSMUSG00000007029 (93%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:1000 - 1:2500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Protocols
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Protein Name
valyl-tRNA synthetase
Gene Name
VARS
Alternative Gene Names
VARS2
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000204394 on the Human Protein Atlas

Siekierska A, Stamberger H, Deconinck T, Oprescu SN, Partoens M, Zhang Y, Sourbron J, Adriaenssens E, Mullen P, Wiencek P, Hardies K, Lee JS, Giong HK, Distelmaier F, Elpeleg O, Helbig KL, Hersh J, Isikay S, Jordan E, Karaca E, Kecskes A, Lupski JR, Kovacs-Nagy R, May P, Narayanan V, Pendziwiat M, Ramsey K, Rangasamy S, Shinde DN, Spiegel R, Timmerman V, von Spiczak S, Helbig I, C4RCD Research GroupBalakChrisC23BelnapNewellN23ClaasenAnaA23CourtrightAmandaA23de BothMattM23HuentelmanMatthew J.MJ23NaymikMarcusM23RichholtRyanR23SiniardAshley L.AL23SzelingerSzabolcsS23CraigDavid W.DW32SchrauwenIsabelleI33, AR working group of the EuroEPINOMICS RES ConsortiumAfawiZaidZ34BallingRudiR22BaulacStéphanieS3536373839BarišićNinaN40CaglayanHande S.HS41CraiuDanaD42Guerrero-LópezRosaR43GuerriniRenzoR44HjalgrimHelleH4546JähnJohannaJ24KleinKarl MartinKM47LeguernEricE3536373839LemkeJohannes R.JR48LercheHolgerH49MariniCarlaC44MøllerRikke S.RS4546MuhleHiltrudH24RosenowFelixF47SerratosaJoseJ43SulsArvidA2353StephaniUlrichU24ŠtěrbováKatalinK50StrianoPasqualeP51ZaraFedericoF52, Weckhuysen S, Francklyn C, Antonellis A, de Witte P, De Jonghe P

Biallelic VARS variants cause developmental encephalopathy with microcephaly that is recapitulated in vars knockout zebrafish

Nat Commun , 2019 Feb 12; 10:708. Epub 2019 Feb 12

PubMed ID: 30755616 DOI: 10.1038/s41467-018-07953-w

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers 50% of the product price as a refund, or 50% discount on your next vial purchase.

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Corresponding Antigens

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