Enhanced Validation

Polyclonal Anti-VAMP4 Antibody

vesicle-associated membrane protein 4
References (2)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Polyclonal Antibody against Human VAMP4
Open Datasheet
Price
$505.00
Product Number
HPA050418
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[5 pictures]
Immunohistochemistry analysis in human cerebral cortex and skeletal muscle tissues using HPA050418 antibody. Corresponding VAMP4 RNA-seq data are presented for the same tissues.
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[1 pictures]
Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.

Additional Images

See additional IHC images using this antibody for 44 normal tissues and 20 cancers on the Human Protein Atlas
Target Protein
vesicle-associated membrane protein 4
Target Gene
VAMP4
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
MPPKFKRHLNDDDVTGSVKSERRNLLEDDSDEEEDFFLRGPSGPRFGPRNDK
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000026696 (100%)

Rat ENSRNOG00000003071 (100%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:200 - 1:500

Retrieval method: HIER pH6

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for HPA050418 on the Human Protein Atlas
Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for HPA050418 on the Human Protein Atlas
Protein Name
vesicle-associated membrane protein 4
Gene Name
VAMP4
UniProt ID
O75379
Gene (Ensembl)
ENSG00000117533
Entrez Gene ID
Entrez gene: 8674
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000117533 on the Human Protein Atlas

Postema MM, Grega-Larson NE, Meenderink LM, Tyska MJ

PACSIN2-dependent apical endocytosis regulates the morphology of epithelial microvilli

Mol Biol Cell , 2019 Sep 1; 30(19):2515-2526

PubMed ID: 31390291 DOI: 10.1091/mbc.E19-06-0352

Did we miss your publication?

Have you published using HPA050418? Please let us know and we will be happy to include your reference on this page.

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Corresponding Antigens
PrEST Antigen VAMP4 (APrEST83566)

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