Polyclonal Anti-UBB Antibody

ubiquitin B
References (2)
Recommended Applications
Product Description
Polyclonal Antibody against Human UBB
Alternative Gene Names
FLJ25987, MGC8385
Open Datasheet
Price
$505.00
Product Number
HPA049132
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Show all images
[1 pictures]
Immunohistochemical staining of human urinary bladder shows nuclear and cytoplasmic positivity in urothelial cells.
Show all images
[1 pictures]
Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.

Additional Images

See additional IHC images using this antibody for 44 normal tissues and 20 cancers on the Human Protein Atlas
Target Protein
ubiquitin B
Target Gene
UBB
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
GMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEG
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000019505 (100%)

Rat ENSRNOG00000057823 (100%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:200 - 1:500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)
All IHC characterization data in 44 normal tissues and 20 cancers for HPA049132 on the Human Protein Atlas
Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Protein Name
ubiquitin B
Gene Name
UBB
Alternative Gene Names
FLJ25987, MGC8385
UniProt ID
P0CG47
Gene (Ensembl)
ENSG00000170315
Entrez Gene ID
Entrez gene: 7314
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000170315 on the Human Protein Atlas

Guo YN, Dong H, Ma FC, Huang JJ, Liang KZ, Peng JL, Chen G, Wei KL

The clinicopathological significance of decreased miR-125b-5p in hepatocellular carcinoma: evidence based on RT-qPCR, microRNA-microarray, and microRNA-sequencing

Int J Clin Exp Pathol , 2019 Jan 1; 12(1):21-39. Epub 2019 Jan 1

PubMed ID: 31933718

Did we miss your publication?

Have you published using HPA049132? Please let us know and we will be happy to include your reference on this page.

Read more

Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

Read more

Corresponding Antigens
PrEST Antigen UBB (APrEST89098)

Questions?

Our customer support and expert scientific support teams are here to help you succeed in your research.

Get in touch