Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000029833 (94%)
Rat ENSRNOG00000013251 (92%)
Dilution: 1:20 - 1:50
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Genetic validation in WB by siRNA knockdown.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Ubiquitylome analysis identifies dysregulation of effector substrates in SPOP-mutant prostate cancer
Science , 2014 Oct 3; 346(6205):85-89. Epub 2014 Oct 2
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