Enhanced Validation

Anti-TJP1 Antibody

tight junction protein 1
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Polyclonal Antibody against Human TJP1
Alternative Gene Names
DKFZp686M05161, MGC133289, ZO-1
Price
$450.00
Product Number
HPA001637
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Target Protein
tight junction protein 1
Target Gene
TJP1
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
ASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGR
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000030516 (100%)

Rat ENSRNOG00000011077 (99%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:50 - 1:200

Retrieval method: HIER pH6

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Protein Name
tight junction protein 1
Gene Name
TJP1
Alternative Gene Names
DKFZp686M05161, MGC133289, ZO-1
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000104067 on the Human Protein Atlas

Zhang C, Mao HL, Cao Y

Nuclear accumulation of symplekin promotes cellular proliferation and dedifferentiation in an ERK1/2-dependent manner

Sci Rep , 2017 Jun 19; 7:3769. Epub 2017 Jun 19

PubMed ID: 28630428 DOI: 10.1038/s41598-017-04005-z

González-Andrades M, Garzón I, Gascón MI, Muñoz-Avila JI, Sánchez-Quevedo MC, Campos A, Alaminos M

Sequential development of intercellular junctions in bioengineered human corneas.

J Tissue Eng Regen Med , 2009 Aug; 3(6):442-9

PubMed ID: 19415764 DOI: 10.1002/term.178

Gangl K, Reininger R, Bernhard D, Campana R, Pree I, Reisinger J, Kneidinger M, Kundi M, Dolznig H, Thurnher D, Valent P, Chen KW, Vrtala S, Spitzauer S, Valenta R, Niederberger V

Cigarette smoke facilitates allergen penetration across respiratory epithelium.

Allergy , 2009 Mar; 64(3):398-405. Epub 2008 Dec 16

PubMed ID: 19120070 DOI: 10.1111/j.1398-9995.2008.01861.x

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Alternative Antibodies
Corresponding Antigens

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