Dilution: 1:1000 - 1:2500
Retrieval method: HIER pH6
Working concentration: 0.4 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Recombinant expression validation in WB using target protein overexpression.
Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
In situ protein detection with enhanced specificity using DNA-conjugated antibodies and proximity ligation
Modern Pathology , September 22, 2017
Dynamics of Protein Expression Reveals Primary Targets and Secondary Messengers of Estrogen Receptor Alpha Signaling in MCF-7 Breast Cancer Cells
Mol Cell Proteomics , 2016 Apr 11; 15(6):2093-2107. Epub 2016 Apr 11
Antibody-based Protein Profiling of the Human Chromosome 21
Mol Cell Proteomics , 2012 Mar; 11(3):M111.013458. Epub 2011 Oct 31
Gene expression signatures differentiate ovarian/peritoneal serous carcinoma from breast carcinoma in effusions.
J Cell Mol Med , 2011 Mar; 15(3):535-44. Epub 2010 Jan 28
Candidate Serological Biomarkers for Cancer Identified from the Secretomes of 23 Cancer Cell Lines and the Human Protein Atlas
Mol Cell Proteomics , 2010 Jun; 9(6):1100-1117. Epub 2010 Feb 1
The Human Protein Atlas--a tool for pathology.
J Pathol , 2008 Dec; 216(4):387-93
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