Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
Lane 4: Human plasma (IgG/HSA depleted)
Lane 5: Human liver tissue
Lane 6: Human tonsil tissue
Highest antigen sequence identity to the following orthologs:
Rat ENSRNOG00000025233 (53%)
Mouse ENSMUSG00000041359 (52%)
Dilution: 1:200 - 1:500
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
The Transcriptomic and Proteomic Landscapes of Bone Marrow and Secondary Lymphoid Tissues
PLoS One , 2014; 9(12):e115911. Epub 2014 Dec 26
IGHV3-21 gene usage is associated with high TCL1 expression in chronic lymphocytic leukemia.
Eur J Haematol , 2010 Feb 1; 84(2):109-16. Epub 2009 Nov 3
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