Dilution: 1:200 - 1:500
Retrieval method: HIER pH6
Working concentration: 0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 1-4 µg/ml
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy.
J Proteomics , 2012 Apr 3; 75(7):2236-51. Epub 2012 Feb 15
The tumour-suppressive function of miR-1 and miR-133a targeting TAGLN2 in bladder cancer
Br J Cancer , 2011 Mar 1; 104(5):808-818. Epub 2011 Feb 8
miR-1 as a tumor suppressive microRNA targeting TAGLN2 in head and neck squamous cell carcinoma
Oncotarget , 2011 Jan-Feb; 2(1-2):29-42. Epub 2011 Jan 25
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