Enhanced Validation

Anti-TAGLN Antibody

Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Polyclonal Antibody against Human TAGLN
Alternative Gene Names
DKFZp686P11128, SM22, SMCC, TAGLN1, WS3-10
Product Number
Unit Size
Lot dependent
2 in Stock
Ready to Ship
Target Protein
Target Gene
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Verified Species Reactivity
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000032085 (97%)

Rat ENSRNOG00000017628 (96%)

40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:200 - 1:500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Protein Name
Gene Name
Alternative Gene Names
DKFZp686P11128, SM22, SMCC, TAGLN1, WS3-10
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Normally shipped at ambient temperature
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000149591 on the Human Protein Atlas

Chen Z, He S, Zhan Y, He A, Fang D, Gong Y, Li X, Zhou L

TGF-β-induced transgelin promotes bladder cancer metastasis by regulating epithelial-mesenchymal transition and invadopodia formation

EBioMedicine , 2019 Aug 13; 47:208-220. Epub 2019 Aug 13

PubMed ID: 31420300 DOI: 10.1016/j.ebiom.2019.08.012

Kryza T, Silva LM, Bock N, Fuhrman‐Luck RA, Stephens CR, Gao J, Samaratunga H, Australian Prostate Cancer BioResource, Lawrence MG, Hooper JD, Dong Y, Risbridger GP, Clements JA

Kallikrein‐related peptidase 4 induces cancer‐associated fibroblast features in prostate‐derived stromal cells

Mol Oncol , 2017 Aug 10; 11(10):1307-1329. Epub 2017 Aug 10

PubMed ID: 28510269 DOI: 10.1002/1878-0261.12075

Huipeng Li, Elise T Courtois, Debarka Sengupta, Yuliana Tan, Kok Hao Chen, Jolene Jie Lin Goh, Say Li Kong, Clarinda Chua, Lim Kiat Hon, Wah Siew Tan, Mark Wong, Paul Jongjoon Choi, Lawrence J K Wee, Axel M Hillmer, Iain Beehuat Tan, Paul Robson, Shyam Prabhakar

Reference component analysis of single-cell transcriptomes elucidates cellular heterogeneity in human colorectal tumors

Nature Genetics , 49, 708-718 (2017)

PubMed ID: None DOI: 10.1038/ng.3818

Claudio Isella, Andrea Terrasi, Sara Erika Bellomo, Consalvo Petti, Giovanni Galatola, Andrea Muratore, Alfredo Mellano, Rebecca Senetta, Adele Cassenti, Cristina Sonetto, Giorgio Inghirami, Livio Trusolino, Zsolt Fekete, Mark De Ridder, Paola Cassoni, Guy Storme, Andrea Bertotti, Enzo Medico

Stromal contribution to the colorectal cancer transcriptome

Nature Genetics , February 23, 2015

PubMed ID: 0 DOI: 10.1038/ng.3224

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Corresponding Antigens


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