Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000032085 (97%)
Rat ENSRNOG00000017628 (96%)
Dilution: 1:200 - 1:500
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
TGF-β-induced transgelin promotes bladder cancer metastasis by regulating epithelial-mesenchymal transition and invadopodia formation
EBioMedicine , 2019 Aug 13; 47:208-220. Epub 2019 Aug 13
Kallikrein‐related peptidase 4 induces cancer‐associated fibroblast features in prostate‐derived stromal cells
Mol Oncol , 2017 Aug 10; 11(10):1307-1329. Epub 2017 Aug 10
Reference component analysis of single-cell transcriptomes elucidates cellular heterogeneity in human colorectal tumors
Nature Genetics , 49, 708-718 (2017)
Stromal contribution to the colorectal cancer transcriptome
Nature Genetics , February 23, 2015
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