Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000038486 (96%)
Rat ENSRNOG00000021182 (96%)
Dilution: 1:200 - 1:500
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Levetiracetam treatment ameliorates LRRK2 pathological mutant phenotype
J Cell Mol Med , 2019 Sep 27; 23(12):8505-8510. Epub 2019 Sep 27
Lipid-dependent deposition of alpha-synuclein and Tau on neuronal Secretogranin II-positive vesicular membranes with age
Sci Rep , 2018 Oct 12; 8:15207. Epub 2018 Oct 12
Amyloid Beta A4 Precursor Protein-binding Family B Member 1 (FE65) Interactomics Revealed Synaptic Vesicle Glycoprotein 2A (SV2A) and Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 2 (SERCA2) as New Binding Proteins in the Human Brain
Mol Cell Proteomics , 2014 Feb; 13(2):475-488. Epub 2013 Nov 27
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Nat Methods , 2013 Apr; 10(4):315-23. Epub 2013 Feb 24
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