Enhanced Validation

Anti-SUN2 Antibody

Sad1 and UNC84 domain containing 2
Recommended Applications
Genetic validation in WB by siRNA knockdown.
Product Description
Polyclonal Antibody against Human SUN2
Alternative Gene Names
UNC84B
Price
$450.00
Product Number
HPA001209
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Target Protein
Sad1 and UNC84 domain containing 2
Target Gene
SUN2
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
LKSEWQSMTQESFQESSVKELRRLEDQLAGLQQELAALALKQSSVAEEVGLLPQQIQAVRDDVESQFPAWISQFLARGGGGRVGLLQREEMQAQLRELESKILTHVAEMQGKSAREAAASLSLTLQKEGVIGVTEEQVHHIVKQALQRYSE
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000015177 (80%)

Mouse ENSMUSG00000042524 (78%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:1000 - 1:2500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Protocols
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Protein Name
Sad1 and UNC84 domain containing 2
Gene Name
SUN2
Alternative Gene Names
UNC84B
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000100242 on the Human Protein Atlas

Gilbert HT, Mallikarjun V, Dobre O, Jackson MR, Pedley R, Gilmore AP, Richardson SM, Swift J

Nuclear decoupling is part of a rapid protein-level cellular response to high-intensity mechanical loading

Nat Commun , 2019 Sep 12; 10:4149. Epub 2019 Sep 12

PubMed ID: 31515493 DOI: 10.1038/s41467-019-11923-1

Bonnin E, Cabochette P, Filosa A, Jühlen R, Komatsuzaki S, Hezwani M, Dickmanns A, Martinelli V, Vermeersch M, Supply L, Martins N, Pirenne L, Ravenscroft G, Lombard M, Port S, Spillner C, Janssens S, Roets E, Van Dorpe J, Lammens M, Kehlenbach RH, Ficner R, Laing NG, Hoffmann K, Vanhollebeke B, Fahrenkrog B

Biallelic mutations in nucleoporin NUP88 cause lethal fetal akinesia deformation sequence

PLoS Genet , 2018 Dec 13; 14(12):e1007845. Epub 2018 Dec 13

PubMed ID: 30543681 DOI: 10.1371/journal.pgen.1007845

Yin Y, Zhong J, Li SW, Li JZ, Zhou M, Chen Y, Sang Y, Liu L

TRIM11, a direct target of miR-24-3p, promotes cell proliferation and inhibits apoptosis in colon cancer

Oncotarget , 2016 Nov 24; 7(52):86755-86765. Epub 2016 Nov 24

PubMed ID: 27888625 DOI: 10.18632/oncotarget.13550

Fahrenkrog B, Martinelli V, Nilles N, Fruhmann G, Chatel G, Juge S, Sauder U, Di Giacomo D, Mecucci C, Schwaller J

Expression of Leukemia-Associated Nup98 Fusion Proteins Generates an Aberrant Nuclear Envelope Phenotype

PLoS One , 2016 Mar 31; 11(3):e0152321. Epub 2016 Mar 31

PubMed ID: 27031510 DOI: 10.1371/journal.pone.0152321

Lv XB, Liu L, Cheng C, Yu B, Xiong L, Hu K, Tang J, Zeng L, Sang Y

SUN2 exerts tumor suppressor functions by suppressing the Warburg effect in lung cancer

Sci Rep , 2015/12/10; 5:17940. Epub 2015 Dec 10

PubMed ID: 26658802 DOI: 10.1038/srep17940

Chaumet A, Wright GD, Seet SH, Tham KM, Gounko NV, Bard F

Nuclear envelope-associated endosomes deliver surface proteins to the nucleus

Nat Commun , 2015 Sep 10; 6:8218. Epub 2015 Sep 10

PubMed ID: 26356418 DOI: 10.1038/ncomms9218

Horn HF, Kim DI, Wright GD, Wong ES, Stewart CL, Burke B, Roux KJ

A mammalian KASH domain protein coupling meiotic chromosomes to the cytoskeleton

J Cell Biol , 2013 Sep 30; 202(7):1023-1039

PubMed ID: 24062341 DOI: 10.1083/jcb.201304004

Stadler C, Rexhepaj E, Singan VR, Murphy RF, Pepperkok R, Uhlén M, Simpson JC, Lundberg E

Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.

Nat Methods , 2013 Apr; 10(4):315-23. Epub 2013 Feb 24

PubMed ID: 23435261 DOI: 10.1038/nmeth.2377

Alsarraj J, Faraji F, Geiger TR, Mattaini KR, Williams M, Wu J, Ha NH, Merlino T, Walker RC, Bosley AD, Xiao Z, Andresson T, Esposito D, Smithers N, Lugo D, Prinjha R, Day A, Crawford NP, Ozato K, Gardner K, Hunter KW

BRD4 Short Isoform Interacts with RRP1B, SIPA1 and Components of the LINC Complex at the Inner Face of the Nuclear Membrane

PLoS One , 2013; 8(11):e80746. Epub 2013 Nov 19

PubMed ID: 24260471 DOI: 10.1371/journal.pone.0080746

Gudise S, Figueroa RA, Lindberg R, Larsson V, Hallberg E

Samp1 is functionally associated with the LINC complex and A-type lamina networks.

J Cell Sci , 2011 Jun 15; 124(Pt 12):2077-85

PubMed ID: 21610090 DOI: 10.1242/jcs.078923

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Corresponding Antigens

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