Lane 2: Human cell line RT-4
Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000027315 (78%)
Rat ENSRNOG00000012811 (73%)
Dilution: 1:20 - 1:50
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Dynamics of Protein Expression Reveals Primary Targets and Secondary Messengers of Estrogen Receptor Alpha Signaling in MCF-7 Breast Cancer Cells
Mol Cell Proteomics , 2016 Apr 11; 15(6):2093-2107. Epub 2016 Apr 11
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