Enhanced Validation

Anti-SPINK5 Antibody

serine peptidase inhibitor, Kazal type 5
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Polyclonal Antibody against Human SPINK5
Alternative Gene Names
DKFZp686K19184, FLJ21544, FLJ97536, FLJ97596, FLJ99794, LEKTI, LETKI, NETS, NS, VAKTI
Price
$437.00
Product Number
HPA009067
Unit Size
100 µl
Concentration
Lot dependent
Availability
10 in Stock
Ships
Ready to Ship
Target protein
serine peptidase inhibitor, Kazal type 5
Target gene
SPINK5
Antigen sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
DGRLGCTRENDPVLGPDGKTHGNKCAMCAELFLKEAENAKREGETRIRRNAEKDFCKEYEKQVRNGRLFCTRESDPVRGPDGRMHGNKCALCAEIFKQRFSEENSKTDQN
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000055561 (77%)

Rat ENSRNOG00000048953 (74%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Concentration
Lot dependent
Unit Size
100 µl
Concentration
Lot dependent
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:200 - 1:500

Retrieval method: HIER pH6

Immunofluorescence in cell lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 1-4 µg/ml

Protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Protein Name
serine peptidase inhibitor, Kazal type 5
Gene Name
SPINK5
Alternative Gene Names
DKFZp686K19184, FLJ21544, FLJ97536, FLJ97596, FLJ99794, LEKTI, LETKI, NETS, NS, VAKTI
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000133710 on the Human Protein Atlas

Gry M, Oksvold P, Pontén F, Uhlén M

Tissue specific protein expression in human cells, tissues and organs

J Proteomics Bioinform , September 24, 2010; 3:286- 293

PubMed ID: 0 DOI: 10.4172/jpb.1000153

Eränkö E, Ilander M, Tuomiranta M, Mäkitie A, Lassila T, Kreutzman A, Klemetti P, Mustjoki S, Hannula-Jouppi K, Ranki A

Immune cell phenotype and functional defects in Netherton syndrome

Orphanet J Rare Dis , 2018 Nov 26; 13:213. Epub 2018 Nov 26

PubMed ID: 30477583 DOI: 10.1186/s13023-018-0956-6

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Alternative Antibodies
Corresponding Antigens

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