Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000051910 (97%)
Rat ENSRNOG00000020514 (95%)
Dilution: 1:1000 - 1:2500
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Recombinant expression validation in WB using target protein overexpression.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Oncogenic hijacking of a developmental transcription factor evokes vulnerability toward oxidative stress in Ewing sarcoma
Nat Commun , 2020 May 15; 11:2423. Epub 2020 May 15
Myh7b/miR-499 gene expression is transcriptionally regulated by MRFs and Eos
Nucleic Acids Res , 2012 Aug; 40(15):7303-7318. Epub 2012 May 24
Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy.
J Proteomics , 2012 Apr 3; 75(7):2236-51. Epub 2012 Feb 15
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