Enhanced Validation

Anti-SNAP25 Antibody

synaptosomal-associated protein, 25kDa
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.
Product Description
Polyclonal Antibody against Human SNAP25
Alternative Gene Names
bA416N4.2, dJ1068F16.2, RIC-4, RIC4, SEC9, SNAP, SNAP-25
Price
$450.00
Product Number
HPA001830
Unit Size
100 µl
Concentration
Lot dependent
Availability
5 in Stock
Ships
Ready to Ship
Target Protein
synaptosomal-associated protein, 25kDa
Target Gene
SNAP25
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
SSDAYKKAWGNNQDGVVASQPARVVDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG
Verified Species Reactivity
Human, Mouse
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000006037 (100%)

Mouse ENSMUSG00000027273 (100%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:200 - 1:500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Western Blot (WB)

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

Protein Name
synaptosomal-associated protein, 25kDa
Gene Name
SNAP25
Alternative Gene Names
bA416N4.2, dJ1068F16.2, RIC-4, RIC4, SEC9, SNAP, SNAP-25
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000132639 on the Human Protein Atlas

Huang CJ, Lee CL, Liu CY, Huang SH, Hou JW, Chen YH, Chien CC, Ho CM, Lo WC, Hung KL

Detection of lower levels of SNAP25 using multiple microarray systems and its functional significance in medulloblastoma

Int J Mol Med , 2017 Mar 20; 39(5):1195-1205. Epub 2017 Mar 20

PubMed ID: 28339008 DOI: 10.3892/ijmm.2017.2925

Lindskog C, Korsgren O, Pontén F, Eriksson JW, Johansson L, Danielsson A

Novel pancreatic beta cell-specific proteins: antibody-based proteomics for identification of new biomarker candidates.

J Proteomics , 2012 May 17; 75(9):2611-20. Epub 2012 Mar 21

PubMed ID: 22465717 DOI: 10.1016/j.jprot.2012.03.008

Cardoso TC, Ferrari HF, Garcia AF, Bregano LC, Andrade AL, Nogueira AH

Immunohistochemical approach to the pathogenesis of clinical cases of Bovine Herpesvirus type 5 infections

Diagn Pathol , 2010 Sep 10; 5:57. Epub 2010 Sep 10

PubMed ID: 20831786 DOI: 10.1186/1746-1596-5-57

Mulder J, Björling E, Jonasson K, Wernérus H, Hober S, Hökfelt T, Uhlén M

Tissue Profiling of the Mammalian Central Nervous System Using Human Antibody-based Proteomics

Mol Cell Proteomics , 2009 Jul; 8(7):1612-1622. Epub 2009 Apr 7

PubMed ID: 19351664 DOI: 10.1074/mcp.M800539-MCP200

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Corresponding Antigens

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