Polyclonal Anti-SMARCE1 Antibody
Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000037935 (90%)
Rat ENSRNOG00000010676 (89%)
Dilution: 1:200 - 1:500
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Clear cell meningiomas are defined by a highly distinct DNA methylation profile and mutations in SMARCE1
Acta Neuropathol , 2020 Dec 14; 141(2):281-290. Epub 2020 Dec 14
Loss of SMARCE1 expression is a specific diagnostic marker of clear cell meningioma: a comprehensive immunophenotypical and molecular analysis
Brain Pathol , 2017 Jun 12; 28(4):466-474. Epub 2017 Jun 12
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Nat Methods , 2013 Apr; 10(4):315-23. Epub 2013 Feb 24
Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy.
J Proteomics , 2012 Apr 3; 75(7):2236-51. Epub 2012 Feb 15
Join the Explorer Program
Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.