Highest antigen sequence identity to the following orthologs:
Rat ENSRNOG00000057072 (90%)
Mouse ENSMUSG00000031766 (90%)
Dilution: 1:50 - 1:200
Retrieval method: HIER pH6
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Monkeys mutant for PKD1 recapitulate human autosomal dominant polycystic kidney disease
Nat Commun , 2019 Dec 11; 10:5517. Epub 2019 Dec 11
Hnf4a deletion in the mouse kidney phenocopies Fanconi renotubular syndrome
JCI Insight , 2018 Jul 25; 3(14):e97497. Epub 2018 Jul 25
EED, a member of the polycomb group, is required for nephron differentiation and the maintenance of nephron progenitor cells
Development , 2018 Jul 18; 145(14):dev157149. Epub 2018 Jul 18
Reduced Abd-B Hox function during kidney development results in lineage infidelity
Dev Biol , 2018 Mar 26; 438(2):84-93. Epub 2018 Mar 26
Conserved and Divergent Molecular and Anatomic Features of Human and Mouse Nephron Patterning
J Am Soc Nephrol , 2018 Feb 15; 29(3):825-840. Epub 2018 Feb 15
Affinity Proteomics Reveals Elevated Muscle Proteins in Plasma of Children with Cerebral Malaria
PLoS Pathog , 2014 Apr; 10(4):e1004038. Epub 2014 Apr 17
Contribution from Atlas Antibodies, Immunohistochemistry Quality Control Team — March 23, 2016
Customer planned to use the Anti-SLC12A3 antibody (HPA028748) for IHC in human kidney samples and asked if it is the distal kidney tubules or the collecting ducts that are stained by the antibody?
Atlas Antibodies immunohistochemistry QC team performed IHC staining of kidney sections displaying both the kidney medulla and kidney cortex. The existing images on the Human Protein Atlas for the Anti-SLC12A3 antibody (HPA028748) displays tissue cores from the cortex and not the medulla part of the kidney. Distal kidney tubules are present in the cortex and not in the medulla whereas the collecting ducts could be present both in cortex and medulla. In order to make sure if collecting ducts or distal tubules were stained, a section containing both the medulla and cortex was stained with the Anti-SLC12A3 antibody (HPA028748).
Images show staining of tubules in the cortex part of the kidney and not in the medulla, which supports that the antibody targets distal tubules and not collecting ducts.
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