Enhanced Validation

Anti-SIX1 Antibody

SIX homeobox 1
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Genetic validation in WB by siRNA knockdown.
Product Description
Polyclonal Antibody against Human SIX1
Alternative Gene Names
Product Number
Unit Size
Lot dependent
>10 in Stock
Ready to Ship
Target Protein
SIX homeobox 1
Target Gene
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Verified Species Reactivity
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000051367 (99%)

Rat ENSRNOG00000022777 (99%)

40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:500 - 1:1000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 0.04-0.4 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Protein Name
SIX homeobox 1
Gene Name
Alternative Gene Names
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Normally shipped at ambient temperature
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000126778 on the Human Protein Atlas

Terakawa J, Serna VA, Nair DM, Sato S, Kawakami K, Radovick S, Maire P, Kurita T

SIX1 cooperates with RUNX1 and SMAD4 in cell fate commitment of Müllerian duct epithelium

Cell Death Differ , 2020 Jun 22; 27(12):3307-3320. Epub 2020 Jun 22

PubMed ID: 32572167 DOI: 10.1038/s41418-020-0579-z

Li J, Zhang T, Ramakrishnan A, Fritzsch B, Xu J, Wong EY, Loh YH, Ding J, Shen L, Xu PX

Dynamic changes in cis-regulatory occupancy by Six1 and its cooperative interactions with distinct cofactors drive lineage-specific gene expression programs during progressive differentiation of the auditory sensory epithelium

Nucleic Acids Res , 2020 Jan 20; 48(6):2880-2896. Epub 2020 Jan 20

PubMed ID: 31956913 DOI: 10.1093/nar/gkaa012

Prasad MS, Uribe-Querol E, Marquez J, Vadasz S, Yardley N, Shelar PB, Charney RM, García-Castro MI

Blastula stage specification of avian neural crest

Dev Biol , 2019 Oct 11; 458(1):64-74. Epub 2019 Oct 11

PubMed ID: 31610145 DOI: 10.1016/j.ydbio.2019.10.007

De Lope C, Martín-Alonso S, Auzmendi-Iriarte J, Escudero C, Mulet I, Larrasa-Alonso J, López-Antona I, Matheu A, Palmero I

SIX1 represses senescence and promotes SOX2-mediated cellular plasticity during tumorigenesis

Sci Rep , 2019 Feb 5; 9:1412. Epub 2019 Feb 5

PubMed ID: 30723235 DOI: 10.1038/s41598-018-38176-0

Zhang L, Zhou H, Li X, Vartuli RL, Rowse M, Xing Y, Rudra P, Ghosh D, Zhao R, Ford HL

Eya3 partners with PP2A to induce c-Myc stabilization and tumor progression

Nat Commun , 2018 Mar 13; 9:1047. Epub 2018 Mar 13

PubMed ID: 29535359 DOI: 10.1038/s41467-018-03327-4

Fonseca BF, Couly G, Dupin E

Respective contribution of the cephalic neural crest and mesoderm to SIX1-expressing head territories in the avian embryo

BMC Dev Biol , 2017 Oct 10; 17:13. Epub 2017 Oct 10

PubMed ID: 29017464 DOI: 10.1186/s12861-017-0155-z

Tchieu J, Zimmer B, Fattahi F, Amin S, Zeltner N, Chen S, Studer L


Cell Stem Cell , 2017 Sep 7; 21(3):399-410.e7

PubMed ID: 28886367 DOI: 10.1016/j.stem.2017.08.015

Zhang T, Xu J, Maire P, Xu PX

Six1 is essential for differentiation and patterning of the mammalian auditory sensory epithelium

PLoS Genet , 2017 Sep 11; 13(9):e1006967. Epub 2017 Sep 11

PubMed ID: 28892484 DOI: 10.1371/journal.pgen.1006967

Chao L, Liu J, Zhao D

Increased Six1 expression is associated with poor prognosis in patients with osteosarcoma

Oncol Lett , 2017 Mar 6; 13(5):2891-2896. Epub 2017 Mar 6

PubMed ID: 28521394 DOI: 10.3892/ol.2017.5803

Roellig D, Tan-Cabugao J, Esaian S, Bronner ME

Dynamic transcriptional signature and cell fate analysis reveals plasticity of individual neural plate border cells

eLife , 2017 Mar 29; 6:e21620. Epub 2017 Mar 29

PubMed ID: 28355135 DOI: 10.7554/eLife.21620

Zhang X, Xu R

Six1 expression is associated with a poor prognosis in patients with glioma

Oncol Lett , 2017 Jan 10; 13(3):1293-1298. Epub 2017 Jan 10

PubMed ID: 28454249 DOI: 10.3892/ol.2017.5577

Sakakibara I, Wurmser M, Dos Santos M, Santolini M, Ducommun S, Davaze R, Guernec A, Sakamoto K, Maire P

Six1 homeoprotein drives myofiber type IIA specialization in soleus muscle

Skelet Muscle , 2016 Sep 5; 6(1):30. Epub 2016 Sep 5

PubMed ID: 27597886 DOI: 10.1186/s13395-016-0102-x

Suen AA, Jefferson WN, Wood CE, Padilla-Banks E, Bae-Jump VL, Williams CJ

SIX1 Oncoprotein as a Biomarker in a Model of Hormonal Carcinogenesis and in Human Endometrial Cancer

Mol Cancer Res , 2016 Jun 3; 14(9):849-858. Epub 2016 Jun 3

PubMed ID: 27259717 DOI: 10.1158/1541-7786.MCR-16-0084

Sun SH, Liu D, Deng YT, Zhang XX, Wan DY, Xi BX, Huang W, Chen Q, Li MC, Wang MW, Yang F, Shu P, Wu KZ, Gao QL

SIX1 coordinates with TGFβ signals to induce epithelial-mesenchymal transition in cervical cancer

Oncol Lett , 2016 Jun 28; 12(2):1271-1278. Epub 2016 Jun 28

PubMed ID: 27446426 DOI: 10.3892/ol.2016.4797

Christina G. Towers, Anna L. Guarnieri, Doug S. Micalizzi, J. Chuck Harrell, Austin E. Gillen, Jihye Kim, Chu-An Wang, Michael U.J. Oliphant, David J. Drasin, Michelle A. Guney, Peter Kabos, Carol A. Sartorius, Aik-Choon Tan, Charles M. Perou, Joaquin M. Espinosa, Heide L. Ford

The Six1 oncoprotein downregulates p53 via concomitant regulation of RPL26 and microRNA-27a-3p

Nature Communications , 6, (2015)

PubMed ID: 26687066 DOI: 10.1038/ncomms10077

Adrados I, Larrasa JJ, Galarreta A, López-Antona I, Menéndez C, Abad M, Gil J, Moreno-Bueno G, Palmero I

The homeoprotein SIX1 controls cellular senescence through the regulation of p16INK4A and differentiation-related genes

Oncogene , 2015 Oct 26; 35(27):3485-3494. Epub 2015 Oct 26

PubMed ID: 26500063 DOI: 10.1038/onc.2015.408

Simões-Costa M, Stone M, Bronner ME

Axud1 integrates Wnt signaling and transcriptional inputs to drive neural crest formation

Dev Cell , 2015 Aug 6; 34(5):544-554. Epub 2015 Aug 6

PubMed ID: 26256212 DOI: 10.1016/j.devcel.2015.06.024

Koehler KR, Hashino E

3D mouse embryonic stem cell culture for generating inner ear organoids.

Nat Protoc , 2014 Jun; 9(6):1229-44. Epub 2014 May 1

PubMed ID: 24784820 DOI: 10.1038/nprot.2014.100

Kong J, Zhou X, Liu S, Jin T, Piao Y, Liu C, Lin Z

Overexpression of sineoculis homeobox homolog 1 predicts poor prognosis of hepatocellular carcinoma

Int J Clin Exp Pathol , 2014; 7(6):3018-3027. Epub 2014 May 15

PubMed ID: 25031720

Sakakibara I, Santolini M, Ferry A, Hakim V, Maire P

Six Homeoproteins and a linc-RNA at the Fast MYH Locus Lock Fast Myofiber Terminal Phenotype

PLoS Genet , 2014; 10(5):e1004386. Epub 2014 May 22

PubMed ID: 24852826 DOI: 10.1371/journal.pgen.1004386

Sakakibara I, Santolini M, Ferry A, Hakim V, Maire P

Six homeoproteins and a Iinc-RNA at the fast MYH locus lock fast myofiber terminal phenotype.

PLoS Genet , 2014 May; 10(5):e1004386. Epub 2014 May 22

PubMed ID: 24852826 DOI: 10.1371/journal.pgen.1004386

Jefferson WN, Chevalier DM, Phelps JY, Cantor AM, Padilla-Banks E, Newbold RR, Archer TK, Kinyamu HK, Williams CJ

Persistently Altered Epigenetic Marks in the Mouse Uterus After Neonatal Estrogen Exposure

Mol Endocrinol , 2013 Oct; 27(10):1666-1677. Epub 2013 Sep 3

PubMed ID: 24002655 DOI: 10.1210/me.2013-1211

Smith AL, Iwanaga R, Drasin DJ, Micalizzi DS, Vartuli RL, Tan AC, Ford HL

The miR-106b-25 cluster targets Smad7, activates TGF-β signaling, and induces EMT and tumor initiating cell characteristics downstream of Six1 in human breast cancer.

Oncogene , 2012 Dec 13; 31(50):5162-71. Epub 2012 Jan 30

PubMed ID: 22286770 DOI: 10.1038/onc.2012.11

Ono H, Imoto I, Kozaki K, Tsuda H, Matsui T, Kurasawa Y, Muramatsu T, Sugihara K, Inazawa J

SIX1 promotes epithelial-mesenchymal transition in colorectal cancer through ZEB1 activation.

Oncogene , 2012 Nov 22; 31(47):4923-34. Epub 2012 Jan 30

PubMed ID: 22286765 DOI: 10.1038/onc.2011.646

Le Grand F, Grifone R, Mourikis P, Houbron C, Gigaud C, Pujol J, Maillet M, Pagès G, Rudnicki M, Tajbakhsh S, Maire P

Six1 regulates stem cell repair potential and self-renewal during skeletal muscle regeneration

J Cell Biol , 2012 Sep 3; 198(5):815-832

PubMed ID: 22945933 DOI: 10.1083/jcb.201201050

Iwanaga R, Wang CA, Micalizzi DS, Harrell JC, Jedlicka P, Sartorius CA, Kabos P, Farabaugh SM, Bradford AP, Ford HL

Expression of Six1 in luminal breast cancers predicts poor prognosis and promotes increases in tumor initiating cells by activation of extracellular signal-regulated kinase and transforming growth factor-beta signaling pathways.

Breast Cancer Res , 2012 Jul 5; 14(4):R100. Epub 2012 Jul 5

PubMed ID: 22765220 DOI: 10.1186/bcr3219

Farabaugh SM, Micalizzi DS, Jedlicka P, Zhao R, Ford HL

Eya2 is required to mediate the pro-metastatic functions of Six1 via the induction of TGF-β signaling, epithelial-mesenchymal transition, and cancer stem cell properties.

Oncogene , 2012 Feb 2; 31(5):552-62. Epub 2011 Jun 27

PubMed ID: 21706047 DOI: 10.1038/onc.2011.259

McCoy EL, Iwanaga R, Jedlicka P, Abbey NS, Chodosh LA, Heichman KA, Welm AL, Ford HL

Six1 expands the mouse mammary epithelial stem/progenitor cell pool and induces mammary tumors that undergo epithelial-mesenchymal transition.

J Clin Invest , 2009 Sep; 119(9):2663-77. Epub 2009 Aug 24

PubMed ID: 19726883 DOI: 10.1172/JCI37691

Micalizzi DS, Christensen KL, Jedlicka P, Coletta RD, Barón AE, Harrell JC, Horwitz KB, Billheimer D, Heichman KA, Welm AL, Schiemann WP, Ford HL

The Six1 homeoprotein induces human mammary carcinoma cells to undergo epithelial-mesenchymal transition and metastasis in mice through increasing TGF-beta signaling.

J Clin Invest , 2009 Sep; 119(9):2678-90. Epub 2009 Aug 24

PubMed ID: 19726885 DOI: 10.1172/JCI37815

Wan F, Miao X, Quraishi I, Kennedy V, Creek KE, Pirisi L

Gene expression changes during HPV-mediated carcinogenesis: A comparison between an in vitro cell model and cervical cancer

Int J Cancer , 2008 Jul 1; 123(1):32-40

PubMed ID: 18398830 DOI: 10.1002/ijc.23463

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