Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000051367 (99%)
Rat ENSRNOG00000022777 (99%)
Dilution: 1:500 - 1:1000
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Genetic validation in WB by siRNA knockdown.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
SIX1 cooperates with RUNX1 and SMAD4 in cell fate commitment of Müllerian duct epithelium
Cell Death Differ , 2020 Jun 22; 27(12):3307-3320. Epub 2020 Jun 22
Dynamic changes in cis-regulatory occupancy by Six1 and its cooperative interactions with distinct cofactors drive lineage-specific gene expression programs during progressive differentiation of the auditory sensory epithelium
Nucleic Acids Res , 2020 Jan 20; 48(6):2880-2896. Epub 2020 Jan 20
Blastula stage specification of avian neural crest
Dev Biol , 2019 Oct 11; 458(1):64-74. Epub 2019 Oct 11
SIX1 represses senescence and promotes SOX2-mediated cellular plasticity during tumorigenesis
Sci Rep , 2019 Feb 5; 9:1412. Epub 2019 Feb 5
Eya3 partners with PP2A to induce c-Myc stabilization and tumor progression
Nat Commun , 2018 Mar 13; 9:1047. Epub 2018 Mar 13
Respective contribution of the cephalic neural crest and mesoderm to SIX1-expressing head territories in the avian embryo
BMC Dev Biol , 2017 Oct 10; 17:13. Epub 2017 Oct 10
A MODULAR PLATFORM FOR DIFFERENTIATION OF HUMAN PSCS INTO ALL MAJOR ECTODERMAL LINEAGES
Cell Stem Cell , 2017 Sep 7; 21(3):399-410.e7
Six1 is essential for differentiation and patterning of the mammalian auditory sensory epithelium
PLoS Genet , 2017 Sep 11; 13(9):e1006967. Epub 2017 Sep 11
Increased Six1 expression is associated with poor prognosis in patients with osteosarcoma
Oncol Lett , 2017 Mar 6; 13(5):2891-2896. Epub 2017 Mar 6
Dynamic transcriptional signature and cell fate analysis reveals plasticity of individual neural plate border cells
eLife , 2017 Mar 29; 6:e21620. Epub 2017 Mar 29
Six1 expression is associated with a poor prognosis in patients with glioma
Oncol Lett , 2017 Jan 10; 13(3):1293-1298. Epub 2017 Jan 10
Six1 homeoprotein drives myofiber type IIA specialization in soleus muscle
Skelet Muscle , 2016 Sep 5; 6(1):30. Epub 2016 Sep 5
SIX1 Oncoprotein as a Biomarker in a Model of Hormonal Carcinogenesis and in Human Endometrial Cancer
Mol Cancer Res , 2016 Jun 3; 14(9):849-858. Epub 2016 Jun 3
SIX1 coordinates with TGFβ signals to induce epithelial-mesenchymal transition in cervical cancer
Oncol Lett , 2016 Jun 28; 12(2):1271-1278. Epub 2016 Jun 28
The Six1 oncoprotein downregulates p53 via concomitant regulation of RPL26 and microRNA-27a-3p
Nature Communications , 6, (2015)
The homeoprotein SIX1 controls cellular senescence through the regulation of p16INK4A and differentiation-related genes
Oncogene , 2015 Oct 26; 35(27):3485-3494. Epub 2015 Oct 26
Axud1 integrates Wnt signaling and transcriptional inputs to drive neural crest formation
Dev Cell , 2015 Aug 6; 34(5):544-554. Epub 2015 Aug 6
3D mouse embryonic stem cell culture for generating inner ear organoids.
Nat Protoc , 2014 Jun; 9(6):1229-44. Epub 2014 May 1
Overexpression of sineoculis homeobox homolog 1 predicts poor prognosis of hepatocellular carcinoma
Int J Clin Exp Pathol , 2014; 7(6):3018-3027. Epub 2014 May 15
Six Homeoproteins and a linc-RNA at the Fast MYH Locus Lock Fast Myofiber Terminal Phenotype
PLoS Genet , 2014; 10(5):e1004386. Epub 2014 May 22
Six homeoproteins and a Iinc-RNA at the fast MYH locus lock fast myofiber terminal phenotype.
PLoS Genet , 2014 May; 10(5):e1004386. Epub 2014 May 22
Persistently Altered Epigenetic Marks in the Mouse Uterus After Neonatal Estrogen Exposure
Mol Endocrinol , 2013 Oct; 27(10):1666-1677. Epub 2013 Sep 3
The miR-106b-25 cluster targets Smad7, activates TGF-β signaling, and induces EMT and tumor initiating cell characteristics downstream of Six1 in human breast cancer.
Oncogene , 2012 Dec 13; 31(50):5162-71. Epub 2012 Jan 30
SIX1 promotes epithelial-mesenchymal transition in colorectal cancer through ZEB1 activation.
Oncogene , 2012 Nov 22; 31(47):4923-34. Epub 2012 Jan 30
Six1 regulates stem cell repair potential and self-renewal during skeletal muscle regeneration
J Cell Biol , 2012 Sep 3; 198(5):815-832
Expression of Six1 in luminal breast cancers predicts poor prognosis and promotes increases in tumor initiating cells by activation of extracellular signal-regulated kinase and transforming growth factor-beta signaling pathways.
Breast Cancer Res , 2012 Jul 5; 14(4):R100. Epub 2012 Jul 5
Eya2 is required to mediate the pro-metastatic functions of Six1 via the induction of TGF-β signaling, epithelial-mesenchymal transition, and cancer stem cell properties.
Oncogene , 2012 Feb 2; 31(5):552-62. Epub 2011 Jun 27
Six1 expands the mouse mammary epithelial stem/progenitor cell pool and induces mammary tumors that undergo epithelial-mesenchymal transition.
J Clin Invest , 2009 Sep; 119(9):2663-77. Epub 2009 Aug 24
The Six1 homeoprotein induces human mammary carcinoma cells to undergo epithelial-mesenchymal transition and metastasis in mice through increasing TGF-beta signaling.
J Clin Invest , 2009 Sep; 119(9):2678-90. Epub 2009 Aug 24
Gene expression changes during HPV-mediated carcinogenesis: A comparison between an in vitro cell model and cervical cancer
Int J Cancer , 2008 Jul 1; 123(1):32-40
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