Highest antigen sequence identity to the following orthologs:
Rat ENSRNOG00000031067 (63%)
Mouse ENSMUSG00000027790 (61%)
Dilution: 1:200 - 1:500
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Defining the Celiac Disease Transcriptome using Clinical Pathology Specimens Reveals Biologic Pathways and Supports Diagnosis
Sci Rep , 2019 Nov 7; 9:16163. Epub 2019 Nov 7
Rab25 regulates integrin expression in polarized colonic epithelial cells
Mol Biol Cell , 2013 Mar; 24(6):818-831
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