Enhanced Validation

Anti-SERPINI1 Antibody

serpin peptidase inhibitor, clade I (neuroserpin), member 1
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Polyclonal Antibody against Human SERPINI1
Alternative Gene Names
neuroserpin, PI12
Price
$445.00
Product Number
HPA001565
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Target Protein
serpin peptidase inhibitor, clade I (neuroserpin), member 1
Target Gene
SERPINI1
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
DESEVQIPMMYQQGEFYYGEFSDGSNEAGGIYQVLEIPYEGDEISMMLVLSRQEVPLATLEPLVKAQLVEEWANSVKKQKVEVYLPRFTVEQEIDLKDVLKALGI
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000010248 (95%)

Mouse ENSMUSG00000027834 (95%)

Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Affinity purified using the PrEST antigen as affinity ligand
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:500 - 1:1000

Retrieval method: HIER pH6

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 0.25-2 µg/ml

Protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Protein Name
serpin peptidase inhibitor, clade I (neuroserpin), member 1
Gene Name
SERPINI1
Alternative Gene Names
neuroserpin, PI12
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Human Protein Atlas

This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.

All characterization data for ENSG00000163536 on the Human Protein Atlas

Ramnefjell M, Aamelfot C, Helgeland L, Akslen LA

Low expression of SerpinB2 is associated with reduced survival in lung adenocarcinomas

Oncotarget , 2017 Oct 3; 8(53):90706-90718. Epub 2017 Oct 3

PubMed ID: 29207598 DOI: 10.18632/oncotarget.21456

Matsuda Y, Miura K, Yamane J, Shima H, Fujibuchi W, Ishida K, Fujishima F, Ohnuma S, Sasaki H, Nagao M, Tanaka N, Satoh K, Naitoh T, Unno M

SERPINI1 regulates epithelial–mesenchymal transition in an orthotopic implantation model of colorectal cancer

Cancer Sci , 2016 Mar 28; 107(5):619-628. Epub 2016 Mar 28

PubMed ID: 26892864 DOI: 10.1111/cas.12909

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Alternative Antibodies
Corresponding Antigens

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