Highest antigen sequence identity to the following orthologs:
Rat ENSRNOG00000002640 (91%)
Mouse ENSMUSG00000067006 (89%)
Dilution: 1:200 - 1:500
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.
Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.
Genetic validation in WB by siRNA knockdown.
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Microvesicle Proteomic Profiling of Uterine Liquid Biopsy for Ovarian Cancer Early Detection
Mol Cell Proteomics , 2019 Feb 13; 18(5):865-875. Epub 2019 Feb 13
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