Lane 2: Human cell line HEL
Highest antigen sequence identity to the following orthologs:
Rat ENSRNOG00000010188 (100%)
Mouse ENSMUSG00000038331 (100%)
Dilution: 1:1000 - 1:2500
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 0.25-2 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.
Validated against independent antibody Anti-SATB2 HPA001042.
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
SATB2 induction of a neural crest mesenchyme-like program drives melanoma invasion and drug resistance
eLife , 2021 Feb 2; 10:e64370. Epub 2021 Feb 2
Molecular correlates and prognostic significance of SATB1 expression in colorectal cancer
Diagn Pathol , 2012 Aug 30; 7:115. Epub 2012 Aug 30
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