Dilution: 1:1000 - 1:2500
Retrieval method: HIER pH6
Working concentration: 0.04-0.4 µg/ml
Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein.
Validated against independent antibody Anti-SATB2 HPA029543.
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validated in Western blot using relevant lysates (see Western blot application images above)
This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
Generation of monospecific antibodies based on affinity capture of polyclonal antibodies.
Protein Sci , 2011 Nov; 20(11):1824-35. Epub 2011 Oct 12
SATB2 in combination with cytokeratin 20 identifies over 95% of all colorectal carcinomas.
Am J Surg Pathol , 2011 Jul; 35(7):937-48
Extensive expression of craniofacial related homeobox genes in canine mammary sarcomas.
Breast Cancer Res Treat , 2009 Nov; 118(2):333-43. Epub 2008 Dec 2
From gene expression analysis to tissue microarrays: a rational approach to identify therapeutic and diagnostic targets in lymphoid malignancies.
Mol Cell Proteomics , 2006 Jun; 5(6):1072-81. Epub 2006 Mar 8
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